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《Beijing Journal of Stomatology》 2001-02
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EFFECTS OF BASIC FIBROBLAST GROWTH FACTOR ON THE PROLIFERATION AND ALKALINE PHOSPHATASE EXPRESSION OF HUMAN PERIODONTAL LIGAMENT CELLS.

WT5”BZ] LIU Xiao yong et al.Faculty of Stomatology,Capital University of Medical Science,Beijing 100050 [WT5”HZ]  
Objective:[WT5”BZ]To evaluate the biological effects of basic fibroblast growth factor(bFGF)on the proliferation and alkaline phosphatase(ALP)activity in 5% and 10% FBS cultured human periodontal ligament cells(PDLCs).[WT5”HZ]Methods:[WT5”BZ]Thiazoly blue(MTT)colorimetric assay and ALP activity analysis were used at different time points(the 24th,48th and 72th hours)to determine the proliferation and ALP activity of PDLCs after different doses of bFGF were added into the culture medium.[WT5”HZ]Results:[WT5”BZ]When compared with control group,①10%FBS,5ng/ml and 10ng/ml bFGF at the 24 th ,48 th and 72th hours,significantly promoted the proliferation of PDLCs(P0 01~0 05);5%FBS 10ng/ml bFGF at 24 th and 48 th hours,5ng/ml bFGF at 24 th hours,significantly stimulated the proliferation of PDLCs (P0 01);②10% FBS 1ng/ml bFGF at 48th and 72th hours,5ng/ml bFGF at 24 th ,48 th and 72th hours,10ng/ml bFGF at 48 th hours,significantly increased the ALP activity of PDLCs(P0 01~0 05);5%FBS,10ng/ml bFGF at 24 th and 72th hours significantly increased the ALP activity of PDLCs(P0 05).[WT5”HZ]Conclusion:[WT5”BZ]These results suggested that 5ng/ml and 10ng/ml bFGF may simultaneously promote the proliferation and differentiation of human PDLCs during a period of time under specified condition. [WT5”HZ]
【CateGory Index】: R780.2
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【References】
Chinese Journal Full-text Database 3 Hits
1 ZHOU Dong-qing, LV Ying, LIU Xiao-yong. Capital Medical University School of Stomatology, Beijing 100050, China;Preliminary study of effects of epidermal growth factor on periodontal regeneration of autotransplanted rhesus teeth[J];Beijing Journal of Stomatology;2006-04
2 WANG Yu 1, QIAN Feng Lan 2, WANG Ping 1, YANG Ya Ning 1, HAO Jian Jun 1 1Department of Conservative Densitry, School of Stomatology, Fourth Military Medical University, Xi'an 710033, China, 2Department of Clinical Laboratory, Chengdu Political Fire Brigade Hospital, Chengdu 610042, China;Effects of basic fibroblast growth factor on proliferation and differentiation of human periodontal ligament cells in vitro[J];Journal of The Fourth Military Medical University;2003-23
3 Ji Jianxin, Liao Weijiao, Qiu Zhihui, et al. Department of Stomatology, the First Affiliated Hospital, Guangzhou Medical College, Guangzhou 510120, China;Effect of transforming growth factor-α,β and basic fibroblast growth factor on the fibronectin and alkaline phosphatase activity in human periodontal ligament cells in vitro[J];Guangzhou Medical Journal;2005-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 HOU Wei,QIAN Dongmeng, YAN Zhiyong, et al\ Department of Microbiology, Qingdao University Medical College, Qingdao 266021\;;CONSTRUCTION OF RECOMBINANT CLONE OF SITE-SPECIFIC GENE MUTANT OF HUMAN INTERLEUKIN-2[J];Acta Acadimae Medicinae Qingdao;2003-01
2 GUO Jian, SONG Da-lin, WANG Yan-ming (Laboratory of Endocrinology, Qingdao Hiser Medical Group, Qingdao 266033, China);;ESTABLISHMENT OF MODEL IN DIABETES MELLITUS COMPLICATED WITH INFECTION AND CHANGES OF TUMOR NECROSIS FACTOR α IN RATS[J];Acta Acadimae Qingdao Umvesitatis;2005-01
3 MENG LIN,LIU MING-JUN,WANG BIN, et al(Department of Microbiology,Qingdao University Medical College,Qingdao 266021,China);CLONING AND PROKARYOTIC EXPRESSION AND ACTIVITY OF ~(88)Arg IL-2[J];Acta Academiae Medicinae Qingdao Universitatis;2006-04
4 CHEN Li-Long,ZHU Xiao-Tong,JIANG Qing-Yan **,GAO Ping,SHU Gang,FU Wei-LongCollege of Animal Science, South China Agricultural University, Guangzhou 510642, China;Effects of synthetic bursin on proliferation of chicken splenic lymphocytes and expression of IL-2 and IL-6[J];Acta Zoologica Sinica;2006-01
5 CHEN Cui-li~1,SHI Ding-ji~2(1.College of Arts and Science of Beijing Union University,Beijing 100083,China;2.China Institute of Botany,The Chinese Academy of Science, Beijing 100093,China);Construction of Anabaena Transferred the Gene Encoding the Human Granulocyte Colony-stimulating Factor[J];Journal of Beijing Union University;2005-03
6 CHEN Su-juan, DONG Xiao-jun, SUN Xiao-li, et al. (Centre of Science and Technique Development, General Hospital of PLA, Beijing 100853, China);Establishment and Primary Application of Interleukin-4 Radioimmunoassay[J];Labeled Immunoassays and Clinical Medicine;2002-01
7 NIU Ying-peng(College of Physical Education Science of Henan University,Kaifeng 475001,Henan China);The Effect of EGb on the Activities of CK,LDH and IL-4 in Blood of Rats[J];Journal of Beijing Sport University;2006-10
8 Yao Huiqin,Wang Yang,Zheng Jie,et al (Beijing Tongren Hospital,Beijing 100730);Study on levels of IL-8 in serum and urine of patients with acute urinary tract infection[J];Beijing Medical Journal;2001-01
9 LI Yan,DU Jin-kai,LI Ru-hong,et al (Affiliated Hospital of Chengde Medical College, Hebei Chengde 067000,China);STUDY ON THE EXPRESSIVE CHANGES OF BFGF IN HYPERTENSIVE RAT’S CARDIAC MUSCLE CELLS DURING DIFFERENT PHASES[J];Journal of Chengde Medical College;2004-01
10 ZHAO Xiang-yang, LI Yu-hong, XU Qian, et al(266 Hospital of Chinese People's Liberation Army, Hebei Chengde 067000, China);STUDY ON THE EXPRESSION OF IL-6, IL-6R AND APOPTOSIS-ASSOCIATED GENES IN GASTRIC CARCINOMA[J];Journal of Chengde Medical College;2005-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 YANG Hai-ling et al.Faculty of Stomatology,Capital University of Medical Sciences,Beijing 100050;EFFECTS OF EPIDERMAL GROWTH FACTOR ON BALB/3T3 FIBROBLAST CELL-CYCLE AND EXPRESSION OF EPIDERMAL GROWTH FACTOR RECEPTOR[J];Beijing Journal of Stomatology;2004-03
2 SHI Guixin 1,WANG Shenguo 1,BEI Jianzhong 1,XIA Zhaofan 2,XIAO Shichu 2,LIU Wang 2.1 Center for Molecular Sciences,Institute of Chemistry,Chinese Academy of Science,Beijing\ 100080;2 The Second Military Medical University,Shanghai\ 200433;Skin Tissue Engineering,Fabrication and Biocompatibility Evaluation of Cell Scaffold[J];Beijing Biomedical Engineering;2002-03
3 LIU Hong1, WU Zhi Feng 2, WANG Qin Tao 2, ZHOU Bin 3 (1. Department of Stomatology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China; 2. Department of Periodontology, School of Stomatology, Fourth Military Medical Univers;Effect of insulin-like growth factor-Ⅰ on osteocalcin secretion of periodontal ligament cells[J];Academic Journal of Second Military Medical University;2001-05
4 Wang Jun 1,2 Li Xinsong 1,2 Zhao Yanqiu 3 Pu Yuepu 3 ( 1Department of Chemistry and Chemical Engineering, Southeast University, Nanjing 210096, China) ( 2Laboratory for Biomaterials and Drug Delivery, Southeast University, Nanjing 210096, China) ( 3Public Health College, Southeast University, Nanjing 210096, China);Preparation and biological properties of porous chitosan scaffolds[J];Journal of Southeast University (Natural Science Edition);2004-01
5 Chen Ping;Han Benli;Li Kun;Duan Henchun (Department of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University,Chongqing, 400038);The effects of activated Kupffer cells on liver cell regeneration in cirrhotic rats[J];ACTA ACADEMIAE MEDICINE MILITARIS TERTIAE;1998-04
6 HE Fei, TAN Ying hui, YANG Zheng rong (Department of Stomatology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China);Consecutive culture of human dental pulp cells in vitro[J];Acta Academiae Medicine Militaris Tertiae;2002-07
7 WANG Yu 1, QIAN Feng Lan 2, WANG Ping 1, YANG Ya Ning 1, HAO Jian Jun 1 1Department of Conservative Densitry, School of Stomatology, Fourth Military Medical University, Xi'an 710033, China, 2Department of Clinical Laboratory, Chengdu Political Fire Brigade Hospital, Chengdu 610042, China;Effects of basic fibroblast growth factor on proliferation and differentiation of human periodontal ligament cells in vitro[J];Journal of The Fourth Military Medical University;2003-23
8 ZHENG Lei WANG Qian WEI Kuan-hai Pei Guo-xian (Department of Orthopedics and Traumatology. Clinical Laboratory Nanfang Hospital, First Military Medical University. Guangzhou 510515. China);Biological effects of basic fibroblast growth factor on bone marrow stromal cells[J];JOURNAL OF FIRST MILITARY MEDICAL UNIVERSITY;2000-02
9 Fan Weihua, et al. Guang Dong Provincial Stomatological Hospital, Guangzhou 510280;Effect of Acid and Inacid Root Conditioning Agents on Root Surfaces.Ⅰ Ultrastructure Observation.[J];Journal of Dental Prevention and Treatment;2003-02
10 XU Jin-jun, et al. College of Stomatology, Hubei Medical University, Wuhan,430070;Immunolocalization of Basic Fibroblast Growth Factor(bFGF) in Healthy and Inflamed Dental Pulp.[J];JOURNAL OF DENTAL PREVENTION AND TREATMENT;1999-03
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 TANG Xin-bao~1,LIU Jing~2(1.Department of Orthodontia,Hospital of Stomatology of Changsha,Changsha 410005,China;2.Department of Stomatology,Medical College,Jinan University,Guangzhou 510632,China);Changes of basic fibroblast growth factor in periodontal tissue during orthodontic tooth movement in young rat[J];Journal of Jinan University(Natural Science & Medicine Edition);2005-06
2 YU Shan-shan,JIANG Xin-quan,WENG Yu-lai,HUA Fei.(Department of Oral Medicine,Ninth People's Hospital,School of Medicine,Shanghai Jiaotong University,Shanghai Key Lab of Stomatology,Shanghai Institute of Stomatology,Shanghai 200011,China);Effects of acidic fibroblast growth factor and transforming growth factor-β1 on proliferation of porcine dental papilla cells[J];Stomatology;2007-07
3 ZHOU Yu-peng The Factory Worker Hospital of Harbin,Harbin150050,China;Anterior Dislocation of Replantation Effect Entirely Related Factors Stuty[J];Aerospace Medicine;2009-08
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