EFFECTS OF BASIC FIBROBLAST GROWTH FACTOR ON THE PROLIFERATION AND ALKALINE PHOSPHATASE EXPRESSION OF HUMAN PERIODONTAL LIGAMENT CELLS.

WT5”BZ] LIU Xiao yong et al.Faculty of Stomatology,Capital University of Medical Science,Beijing 100050 [WT5”HZ]  

Objective:[WT5”BZ]To evaluate the biological effects of basic fibroblast growth factor(bFGF)on the proliferation and alkaline phosphatase(ALP)activity in 5% and 10% FBS cultured human periodontal ligament cells(PDLCs).[WT5”HZ]Methods:[WT5”BZ]Thiazoly blue(MTT)colorimetric assay and ALP activity analysis were used at different time points(the 24th,48th and 72th hours)to determine the proliferation and ALP activity of PDLCs after different doses of bFGF were added into the culture medium.[WT5”HZ]Results:[WT5”BZ]When compared with control group,①10%FBS,5ng/ml and 10ng/ml bFGF at the 24 th ,48 th and 72th hours,significantly promoted the proliferation of PDLCs(P0 01～0 05);5%FBS 10ng/ml bFGF at 24 th and 48 th hours,5ng/ml bFGF at 24 th hours,significantly stimulated the proliferation of PDLCs (P0 01);②10% FBS 1ng/ml bFGF at 48th and 72th hours,5ng/ml bFGF at 24 th ,48 th and 72th hours,10ng/ml bFGF at 48 th hours,significantly increased the ALP activity of PDLCs(P0 01～0 05);5%FBS,10ng/ml bFGF at 24 th and 72th hours significantly increased the ALP activity of PDLCs(P0 05).[WT5”HZ]Conclusion:[WT5”BZ]These results suggested that 5ng/ml and 10ng/ml bFGF may simultaneously promote the proliferation and differentiation of human PDLCs during a period of time under specified condition. [WT5”HZ]

【CateGory Index】： R780.2

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