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《Beijing Journal of Stomatology》 2007-02
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Effect of hyperthermia on multidrug resistance of tongue squamous cell carcinoma cell line Tca 8113

LENG Wei-dong,WANG Da-zhang.Department of Stomatology,Taihe Hospital,Yunyang Medical College,Shiyan 442000,China  
Objective To study the effect of hyperthermia on expression of multidrug resistance-related(MDR) proteins and intracellular ADM concentration in tongue squamous cell carcinoma cell line Tca 8113 and its multidrug resistance(MDR) cell line Tca8113/CBDEA.MethodsCell lines were hyperthermia-treated at 42 ℃ for 0.5h,and 4 h and 24 h later,expression of MDR-related proteins(P-gp,MRP1,GST-π)were examined using immunohistochemistry.HTS 7000 Plus Bio Assay Reader was used to measure the intracellular ADM concentration.ResultsIn Tca 8113/CBDEA cells,expression of P-gp,MRP,GST-π was down-regulated at 4h posthypertheramia(P0.01).At 24h posthyperthermia,the expression levels of P-gp,MRP,GST-π were lower than those observed at 4h(P0.01).In Tca 8113 cells,P-gp expression did not change(P0.05).MRP1 expression decreased(P0.05),but there was no statistical difference between 4h and 24h posthyperthermia(P0.05).GST-π expression did not change at 4h(P0.05),but decreased at 24h posthypertheramia(P0.05).Intracellular drug(ADM)concentration increased in Tca 8113/CBDEA and Tca 8113(P0.01)cells.The F value of Tca 8113 was 7.58 times that of Tca 8113/CBDEA before hyperthermia,but it was 3.63 and 2.40 folds at 4h and 24h posthyperthermia.ConclusionHyperthermia inhibited the expression of multidrug resistance-related(MDR) proteins and increased the intracellular ADM concentration.The results suggest that hyperthermia could be used as a method to overcome MDR.
【CateGory Index】: R739.8
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