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《JOURNAL OF BEIJING INSTITUTE OF TECHNOLOGY》 1999-06
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Extraction of Antitumor Component from Fruiting Body of Ganoderma Lucidum

Zhao Dongxu Wang Libo Yang Xinlin Zhu Hesun (Center for Research on Materials Science, Beijing Institute of Technology, Beijing100081)  
Aim To investigate the factors affecting the extraction of antitumor component from Ganoderma lucidum . Methods After being soaked in ethanol, the antitumor component was extracted by the method of percolation. The orthogonal design with table L 9(3 4) was taken. The relative content of antitumor component in the extract was estimated by its harmfulness to HeLa cell. Results The extract inhibits the growth of HeLa cell in vitro. Among the 4 factors affecting the percolation, the operating temperature was the most significant one associated with the harmfulness and the solvent volume was the least. Conclusion There were some components of inhibiting the growth of Hela cell in the fruiting body of Ganoderma lucidum. The percolating conditions: the soaking time of 1 d, the percolating rate of 0 5 mL·min -1 , the solvent volume of 200 mL and the temperature at 60 ℃, can ensure obtaining the best result of extraction.
【Fund】: 国家科技部基金
【CateGory Index】: R284.2
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