Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Beijing Institute of Technology》 2004-09
Add to Favorite Get Latest Update

Immobilization of Antithrombin-Ⅲ on Fibroin/Heparin Blend Membrane and Its Anticoagulant Performance

YANG Xin-lin~1,WU Ke-you~1,YU Qun~2,FAN Cui-hong~1,LI Mao-lin~1,ZHU He-sun~3 (1.School of Life Science and Technology, Beijing Institute of Technology, Beijing100081, China; 2.Institute of Transfusion Medicine, Academy of Military Medical Sciences, Beijing100850, China; 3.School of Material Science and Engineering, Beijing Institute of Technology, Beijing100081, China)  
A biomaterial silk fibroin is surface modified using antithrombin-Ⅲ(AT-Ⅲ) to improve its anticoagulant property. AT-Ⅲ was immobilized on the surface of silk fibroin/heparin blended membrane by NH_3 plasma treatment, followed by covalent cross-linking reaction. The immobilization efficiency was evaluated by protein surplus analysis method. The in vitro antithrombogenicity representing the activity of AT-Ⅲ was determined by the method of activated partial thromboplastin time(APTT), prothrombin time (PT) and thrombin time (TT) test. The cytotoxicity of materials was tested by the MTT method. The results demonstrated that the AT-Ⅲ is immobilized on the membrane in an efficient and activity preservant form and, the anticoagulant performance of the modified fibroin membrane improved. These results anticipater the wider application of AT-Ⅲ, and provide new visions on the design of anticoagulant materials.
【Fund】: 国家"九七三"计划项目(G1999064705)
【CateGory Index】: R318.08
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 5 Hits
1 Wang Wenxu,Cai Jin,Cen Peilin Zhejiang University,Hangzhou,310027;XPS Investigation of Ammonia Plasma Modified Polypropylene Membrane Surface[J];MATERIALS SCIENCE AND ENGINEERING;1997-02
2 Liu Yongcheng,Shao Zhengzhong,Sun Yuyu,Yu Tongyin(The Laboratory of Molecular Engineering of Polymers,Department of Macromolecular Science,Fudan University,Shanghai 200433);The Structure and Function of Silk Fibroin[J];POLYMER BULLETIN;1998-03
3 Gu Jinwei, Yang Xinlin, Wang Liyong, Zhu Hesun (Research Center of Materials Science, Beijing Institute of Technology, Beijing 100081);Surface Sulfonation of Silk Fibroin Film by Plasma Treatment and in vitro Antithrombogenicity[J];High Technology Letters;2001-08
4 Gao Yunhua, Yang Xinlin, Fan Cuihong, Li Maolin, Zhu Hesun (School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081);Immobilization of v Wf Antibody on Fibroin Membrane and Its AnticoagulantPerformance in vitro[J];High Technology Letters;2003-03
5 Wang Wei Wang Yishou;Research Progress on Antithrombin Ⅲ[J];Shanghai Chemcal Industry;1999-20
Chinese Journal Full-text Database 10 Hits
1 CAO Hai-jian Qian Kun (School of Textile and Garment Southern Yangtze University,Wuxi 214063,China);Development and status quo of vascular prosthesis[J];Beijing Textile Journal;2004-06
2 HUANG Fuhua,ZHENG Jun,SUN Lizhong Department of Cardiac Sugery,Cardiovascular Institute and Fuwai Hospital,CAMS&PUMC,Beijing 100037;In vitro characterization of a new vascular prosthesis coated with silk fibroin[J];Beijing Biomedical Engineering;2008-04
3 Wang Qinmei , Pan Shirong, Zheng Zhensheng (Sun Yetsen University of Medical Sciences, Guagzhou, 510080);Study of Platelet Adhesion on PU Surface with Heparinization[J];BEIJING BIOMEDICAL ENGINEERING;1997-01
4 PAN Zhi-juan,QIU Xin-wei,LI Chun-ping,WANG Jian-min,MIAO Heng-yun(College of Material Engineering,Soochow University,Suzhou 215021,China);Electrospun Regenerated Silk Fibroin Nanofibers: the Fiber Diameter Distributions and Mechanical Properties[J];Journal of Materials Science and Engineering;2006-02
5 LU Qiang CAO Chuanbao ZHU Hesun (Research Center of Materials Science, Beijing Institute of Technology, Beijing 100081);Preparation and blood compatibility of polyurethane films blending and grafting with heparin in the same solvent system[J];Chinese Journal of Materials Research;2004-03
6 Medicine Release Control Zhang Youzhu Wang Zhaoxia Ding Yue Lu Shenzhou Xu Guoyin (Silk College, Suzhou University,Suzhou 215021);Research on Fibroin Protein Membrane as Materialof[J];ACTA SERICOLOGICA SINICA;1999-03
7 Liu Pengl Chen Yashao School of Chemistry and Materials Science, Shanxi Normal University, Xi'an 710062, China;Surface Sulfonation of Polyvinyl Chloride by Plasma for Antithrombogenicity[J];等离子体科学和技术(英文版);2004-03
8 XIONG Meng, WANG Yang, AI Yu Feng, ZHANG Lin Xi Plastic Surgery Center, Xijing Hospital, Fourth Military Medical University, Xi’an 710033,China;Vascular model with tubular polyglycolic acid scaffold precoated with collagen[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;1999-11
9 XU Yi-min1, QI Song-tao1, ZENG Shao-wen2, SHI Xiao-feng1, LI Wei-qiu3, HUANG Guang-long1, ZHUANG Bing-rong3 1Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; 2Central Hospital of Shantou City, 3Center Lab, Shantou University of Medical College, Shantou 515031, China;Optical and scanning electron microscopic observation of a novel small-caliber vascular graft in canine models[J];Journal of Southern Medical University;2007-06
10 HUANG Xi,LI Min~*(Bioengineering College, Fujian Normal University, Fuzhou 350007,China);Process Techniques and Application of Silk Protein Film[J];Journal of Fujian Normal University(Natural Science);2005-02
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Wang Xiufen\ \ Wang Hao\ \ Cao Chuanbao\ \ Yang Xinlin\ \ Zhu Hesun (Center for Research on Materials Science, Beijing Institute of Technology, Beijing100081);Polyethylene Surface Heparinization by Plasma Initiation and Its Biocompatibility[J];JOURNAL OF BEIJING INSTITUTE OF TECHNOLOGY;1999-03
2 Wang Wenxu,Cai Jin,Cen Peilin Zhejiang University,Hangzhou,310027;XPS Investigation of Ammonia Plasma Modified Polypropylene Membrane Surface[J];MATERIALS SCIENCE AND ENGINEERING;1997-02
3 Liu Jianhong;Yu Tongyin(Department of Macromolecular Science);Graft copolymerization of silk fibers(Ⅱ)──Grafting vinyl monomers onto silk fibers in absence of initiator[J];;1994-04
4 Qian Jianghong; Liu Haiying; Deng Jiaqi (Department of Chemistry, Fudan University , Shanghai 200433) Liu Yongcheng; Yu Tongyin (Department of Macromolecular Science, Fudan Universiry) Zhao Huiling; Hu Dezhen (Center of Instrumental analysis , Fudan Unive;An Amperometric Hydrogen Peroxide Sensor Employing Phenazine Methosulphate as an Electron Shuttle[J];Chinese Journal of Analytieal Chemistry;1996-03
5 Qian Guo-di et al.;A Study on the Chemical Constitution of the Fibroin of Chinese Cultivated Silk[J];Journal of Textile Research;1981-02
6 Zhang Ruilin;Morphology and Breaking Process of Raw Silk during Stretching[J];Journal of Textile Research;1985-09
7 Shao Zheng-zhong, Fang Yue, Yu Tong-yin, Deng Jia-qi(Department of Material Science and Departmment of Chemistry, Fudan University, Shanghai, 200433);A Sensor of Glucose Oxidase Immobilized in Bombyx Mori Silk Fibroin Membrane[J];Chemical Research In Chinese Universities;1991-06
8 SUN Yu-Yu; LIU Yong-Cheng; MA Ming-Hua;SHAO Zheng-Zhong; CHEN Xin; YU Tong-Yin (Dept. of Macromolecular Science, Departmemt of Chem., Fudan Univ., Shanghai, 200433);FT-IR Study of Conformation Transition of Silk Fibroin Induced by Intermolecular Hydrogen Bonds[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1998-01
9 QIAN Jiang-Hong; LIU Yong-Cheng; LIU Hai-Ying; YU Tong-Yin; DENG Jia-Qi (Department of Chemistry, Department of Macrmolecular Science ,Fudan University,Shanghai, 200433);Peroxidase Immobilized in Regenerated silk Fibroin Membrane and Its Application to the Sensor in Organic System[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1995-10
10 YU Tong-Yin;ZHANG Sheng;SHAO Zheng-Zhong (Department of Macromolecular Science and Laboratory of Molecular Engineering of Polymers,Fudan University,Shanghai,200433);Conformation Transition of Silk Fibroin[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1996-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved