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《Journal of Beijing Union University》 2005-03
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Construction of Anabaena Transferred the Gene Encoding the Human Granulocyte Colony-stimulating Factor

CHEN Cui-li~1,SHI Ding-ji~2(1.College of Arts and Science of Beijing Union University,Beijing 100083,China;2.China Institute of Botany,The Chinese Academy of Science, Beijing 100093,China)  
The research is to construct engineering Anabaena transferred the gene encoding the human granulocyte colony-stimulating factor: hG-CSFb gene is cloned into middle vector pRL439 and expression vector pDC-8.The vector pDCG-CSF is introduced into Anabaena sp. by tripjarental conjugative transfer .The transformation is confirmed by PCR amplification.
【CateGory Index】: Q943.2
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【References】
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1 XI Chao1 WANG Chun-mei1 SHI Ding-ji2,3 (1 Department of Biopharmaceuticals,School of Materia Medica,Beijing University of Chinese Medicine,Beijing 100102,China) (2 School of Marine Science and Engineering,Tianjin University of Science and Technology,Tianjin 300450,China) (3 Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China);Advances on Cyanobacteria Genetic Engineering Applications[J];China Biotechnology;2010-03
【Citations】
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1 DAI Wei 1.2, SHI Ding-Ji 1 *, ZHANG Hui 1,2, ZHONG Hui 3, RAN Liang 1, PENG Guo-Hong 4, GAN Ren-Bao 5, CHEN Su-Juan 3, LIAN Mu-Lan 2(1. Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China; 2. Department of Biol;Expression of Human Epidermal Growth Factor Gene in Cyanobacteria[J];Acta Botanica Sinica;2001-12
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1 ZHANG Wei CHEN Jin-chun(College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China);Separation and purification of recombinant snake neurotoxins from Pichia pastoris[J];Journal of Beijing University of Chemical Technology;2005-06
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4 ZHAO Jing~(*1,2),TANG Huan-wen~1,ZHU Xun-zhi~1(1.Dept.of Appl.Math.,Dalian Univ.of Technol.,Dalian 116024,China;2.Dept.of Math.and Phys.Sci.,Shandong Inst.of Light Ind.,Jinan 250100,China);An improvement of simulated annealing algorithm and study of its application[J];Journal of Dalian University of Technology;2006-05
5 WEI Lan-zhen(1,2),TAN Wei2,WANG Quan-xi(1,2)(1 School of Life Science,East China Normal University,Shanghai 200062,China;2 College of Life & Environment Sciences,Shanghai Normal University,Shanghai 200234,China);High Expression of hGM-CSF Induced by the cpcβ Gene Promoter in Anabaena sp.PCC 7120[J];Acta Botanica Boreali-Occidentalia Sinica;2008-01
6 PENG Yan,WANG Yong,SONG Fang-Zhou,WANG Ya-PingKey Laboratory of Biochemistry and Molecular Pharmacology,Chongqing University of Medical Sciences,Chongqing 400016,China;Expression and purification of recombinant human IL-18 in pichia pastoris[J];Journal of The Fourth Military Medical University;2005-22
7 CHEN Zhan-hua~(1),YANG Guang-hui~(*,1,2)(1.Educational College of Jilin Province,Changchun 130020,China; 2.State Key Laboratory of Theoretical and Computational Chemistry,Institute of Theoretical Chemistry,Jilin University,Changchun 130023,China);Study on the three-dimensional structure of rat estrogen sulfotransferase[J];Journal of Molecular Science;2006-05
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1 Riming Yan1,2,Zhibin Zhang2,Qinggui Zeng2,Du Zhu2,3,and Ju Chu1 1 State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China 2 Key Laboratory of Protection and Utilization of Subtropical Plant Resources of Jiangxi Province,Jiangxi Normal University,Nanchang 330022,China 3 Yichun University,Yichun 336000,China;Carbon metabolism and energetic utilization of Synechococcus sp.PCC7942 under mixotrophic condition[J];Chinese Journal of Biotechnology;2010-09
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