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《Journal of Beijing Forestry University》 1989-03
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Changes of Nucleic Acids and Proteins in Germination of Chinese pine (Pinus tabulaefermis Carr.) Seed

Zhou Xuequan (Forest Resources College)  
Quantitative changes in the total amount of nucleic acids, DNA, RNA and protein contents in seeds of Chinese pine during germination were found. The most evident changes was in the components of embryonic proteins. Acid proteins with low isoelectric points (pH4.3-5.2) for example increased and accumulated continuously. On the contrary, basic proteins with high isoelectric points (pH6.5-8.0) , and low moleculer weight (4.3×104D) gradually degraded and disappeared. As proved with P.A.S. reaction, they are a group of glycoproteins of different molecular weights. These proteins in the embryo of Chinese pine seeds are characterized by their distinct appearance and disappearance at specific stages of germination. This finding could have far reaching importance in regulating the growth of embryos and the morphogenesis of seedlings.
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