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《Journal of Beijing Forestry University》 1992-03
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Studying Isozymes Gene Markers in Clones of Populus tomentosa Carr.

Zhang Zhiyi Zhu Zhiti(Forest Resources College, BPU)G. Muller-Starck H. H. Hattemer(Gottingen University, Germany)  
Isozymes inheritance and variation were studied in Chinese white poplar (Populus tomentosa Carr. ) by means of electrophoretic technique. The material tested was sampled from clonal archive of Populus tomentosa in Guanxian County, Shandong Province. All plus trees and old trees in the clonal archive were selected and collected from main distribution zone of Populus tomentosa in China. Isozymes from 16 enzyme systems were extracted from six type organ tissues and separated by horizontal starch-gel eletrophoresis. The enzyme systems were genetically stable including eight systems with 12 loci and two systems with 2×5 isozyme patterns. 86 individual plus trees and old trees were classified under 79 distict clones using sets of combined genetic make-up of the ten enzyme systems.Clone overlap rate(CO) was 8.14% and average clone overlap rate(AO) was 1.63%. It was demonstrated that the rich gene resources were conserved in the clonal archive. The ideal numbers and perfect combination of isozyme systems during the analysis were also discussed.
【CateGory Index】: S792.117
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