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《Journal of Beijing Forestry University》 1995-S1
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Tissue Culture of Chimonanthus praecox

Zheng Junbao Zhang Yuman Wang Xuerui (Hebei Forestry College,Baoding 071000)Pei Dong (Institute of Forestry,Chinese Academy of Forestry Science,Beijing 100091)  
Chimonanthus praecox is a famaus Chinese ornamental shrub with high medical value.This paper introduced in detail the process of differentiation,growth,multiplication, and plantlets regeneration during in vitro culture of the plant,using stem tips,segments of tender shoots with lateral buds,cotyledons and embryoes as explants.Meanwhile,the suit- able culture media for various stages were studied.The results showed that dark culture of some period could accelerate the initial differentiation of explants.When adding active char- coal to the media,the browning of explants could be minimized.The calli from stems with leaves were found easy to be induced for differentiation of adventitious buds during culture.
【CateGory Index】: S685.99
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