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《JOURNAL OF BEIJING FORESTRY UNIVERSITY》 2000-05
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Obtaining triploids by high and low temperature treating female flower buds of white poplar.

Li Yun; Zhu Zhiti; Tian Yanting; Zhang Zhiyi; Kang Xiangyang. Journal of Beijing Forestry University (2000)22(5)7~12 [Ch,30 ref.]College of Life Sciences and Biotechnology, Beijing For. Univ., 100083, P.R.China.  
Triploids had been obtained by physical method treating female flower buds of \%P. tomentosa×P. bolleana, P. alba×P. glandulosa, P. alba×P. tomentosa.\% The resultes were as follows: ① It is a moderate treating time for female flower buds on the female flower branches which had been cultured in water for 1~5 days. This period of time was a sensitive phase of female gamete being treated. ② 10 triploids had been obtained by high temperature treatment, the treatment of temperature of 44 ℃ and 40 ℃ had produced 2,8 triploids respectively, the treatment result of 40 ℃ was better. ③ 3 triploids had been obtained by low temperature treatment, the treatment of 4 ℃ and -2 ℃ had produced 1,2 triploids respectively, the treatment of -2 ℃ was better. ④ Treating time was 3,5,7 hours when flower branches which were cultured in water for 1~5 days were treated by the temperature of 40 ℃, 3 hours by the temperature of 44 ℃, 24 hours by the temperature of 4 ℃, and 48 hours by the temperature of -2 ℃. ⑤Triploids have rapid growing, gigas and vigorous properties.
【Fund】: “八五”国家科技攻关加强项目 !(85 18 0 1 0 9)
【CateGory Index】: S792.119
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