Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Beijing Forestry University》 2003-03
Add to Favorite Get Latest Update

DNA template preparation and technology system building for AFLP analysis of Prunus mume.

Ming Jun;Zhang Qixiang;Yan Xiaolan;Qiu Lijuan. Department of Horticulture, Hubei Agricultural College, Jingzhou, 434025, P. R. China.  
The improved method of SDS DNA isolation is used to extract total DNA from tender or old leaves of \%Prunus mume\% in this study. High quality total DNA from plant was isolated in less than 3 hours. The values of OD 260 / OD 280 of each sample were between 1 7 and 1 9 Strips in agarose electrophoresis were clear and less decomposed. DNA samples with high purification and high yield rate could be gained by this method. It is much more simplified than former methods at many steps. The procedure of the method is easy, fast and effective, and is good to extract genomic DNA of \%Prunus mume\%. These genomic DNA samples are intact without contaminating nucleases or inhibitor. The DNA isolated with this method could be completely digested by restriction enzyme of Mse Ⅰ and EcoR Ⅰ, and good enough for use in following applications including PCR and analysis of AFLP. The systems suitable for silver stained AFLP analysis were established and capable of producing sharp fingerprint patterns.
【Fund】: 湖北省自然科学基金资助项目 ( 2 0 0 1ABB118)
【CateGory Index】: S685.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 10 Hits
1 ZHU Hong-xia(Shanghai Polytechnic College of Urban Management,Shanghai 200438 China);DNA Template Preparation and Technology System Construction for AFLP Analysis in Paeonia suffruticosa[J];Northern Horticulture;2007-10
2 GUI Teng-qin1,2,3,QIAO Ai-min3,SUN Min1,WANG Xin-yan3,SUN Xue-mei1(1.College of Life Science,Southwest University,Chongqing 400715,China;2.Dept.of Chemistry & Life Science,Qianxinan Teachers' College,Xingyi,Guizhou 562400,China;3.College of Agriculture and Landscape Architecture,ZhongKai University of Agriculture and Technology,Guangzhou,Guangdong 510225,China);Comparison of Extraction Methods and ISSR Analysis of Genomic DNA in Japanese apricot(Prunus mume Sieb.et Zucc.)[J];Northern Horticulture;2008-04
3 MING Jun; ZHANG Qixiang. College of Horticulture and Landscape Architecture, Yangtze University, Jingzhou, 434025, P. R. China.;AFLP DNA fingerprinting analysis of close genetic relationships of cultivars of Prunus mume Sieb. et Zucc[J];Journal of Beijing Forestry University;2004-05
4 Ming Jun (Institute of vegetables and Flowers, The Chinese Academy of Agricultural Sciences, Beijing, 100081)Zhang Qixiang (College of Landscape Architecture, Beijing Forestry University,100083,P.R. China);Paternity Analysis of Prunus mume‘Meiren’[J];Journal of Beijing Forestry University;2004-S1
5 Liu Yan Zhang Yali (College of Landscape Architecture,Beijing Forestry University,100083,P.R. China);Pollen Cryopreservation of Prunus mume[J];Journal of Beijing Forestry University;2004-S1
6 Liu Qinglin (Department of Landscape Architecture, China Agricultural University, 100094 Beijing,P,R.China);Some Aspects on Research Achievements in Prunus mume in China and Aboad[J];Journal of Beijing Forestry University;2004-S1
7 Li Qingwei (College of Landscape Architecture, Beijing Forestry University,100083,P.R.China);Advances in Mei (Prunus mume) Research[J];Journal of Beijing Forestry University;2004-S1
8 MING Jun 1;ZHANG Qi-xiang 2;LAN Yan-ping 3. 1) Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China; 2) College of Landscape Architecture, Beijing Forestry University, 100083, P. R. China; 3) Institute of Agriculture System and Development, Beijing Academy of Agriculture and Forestry, 100089, P. R. China.;Core collection of Prunus mume Sieb. et Zucc.[J];Journal of Beijing Forestry University;2005-02
9 WANG Zan~(1,2),GAO Hong-wen~2 ,HAN Jian-guo~1(1.Institute of Grassland Science, China Agricultural University, Beijing 100094,China;2.Institute of Animal Science, Chinese Academy of Agricultural Science , Beijing 100094,China);DNA Extraction Method and Profile of AFLP Fingerprint of Caragana korshinskii[J];Acta Agrestia Sinica;2005-02
10 CHEN Hua~1,YI Gan-jun~(2*),XU Xiao-biao~(3*),ZENG Ji-wu~2(1 Department of Biology,Zhaoqing College,Zhaoqing,Guangdong 526061,China;2 Fruit Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;3 Agronomy College,Jiangxi Agricultural University,Nanchang 330045,China);Construction of the AFLP Analysis System for Actinidia Genome[J];Acta Botanica Boreali-occidentalia Sinica;2005-08
Chinese Journal Full-text Database 4 Hits
1 Liu Qinglin (Department of Ornamental Horticulture, China Agricultural University, Beijing, 100094, P.R. China) Chen Junyu (College of Landscape Architecture, Beijing For. Univ., 100083, P.R. China);A Preliminary Research on the Relationship of Prunus mume Cultivars and Its Close Relatives by Using RAPD Assay[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1999-02
2 Yi Ganjun; Huo Heqiang; Cai Changhe; Qiu Yanping; Huang Ziran; Chen Dacheng(1 Pomology Research Institute, Guangdong Academy of Agric. Sci., Guangzhou, 510640;2 Dept. of Sericulture, South China Agric. Univ.;3 Dept. of Horticulture, South China Agric. Uni;DNA Isolation Procedure for AFLP Amplification Reaction of Litchi[J];JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY;1999-03
3 WANG Yan 1 LI Shaoshan 2 LIU Songhao 2 (1 School of Life Science and Technology, Jinan University , Guangzhou 510632) (2.Institute of Laser Life Science, South China Normal University, Guangzhou 510631);Quick Preparation of Total DNA from Plants[J];ACTA LASER BIOLOGY SINICA;2000-01
4 WANG Cai-hong 1,WANG Qian 2,DAI Hong-yi 3,JIA Jian-hang 2,WANG Bin 2,SHU Huai-rui 1 (1 College of Horticulture,Shandong Agricultural University,Taian 271018,China; 2 Genetic Institute of Chinese Academy of Science,Beijing 100101 3 Laiyang Agri;DNA TEMPLATE MAKING AND TECHNOLOGY SYSTEM BUILDING FOR AFLP ANALYSIS OF APPLE GENOME[J];Journal of Shandong Agricultural University;2001-02
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 DU Chuan-yin1,3,4,WANG Yu-jun2,LI Si-shen3,LI Chang-bao3,LIU Hong-xiang4,TIAN Ji-chun3 (1.Weifang Tobacco Limited Corporation,Weifang 261031,China;2.Plant Protection College,Shandong Agricultural University,Taian 271018,China;3.Agronomy College,Shandong Agricultural University,Taian 271018,China;4.Qingzhou Tobacco Research Institute of CNTC,Qingdao 266061,China);Construction of AFLP Silver-staining Analysis System in Tobacco[J];Shandong Agricultural Sciences;2007-05
2 Wang Lianghui1 Si Wei1 Fu Hongtuo1,2 Gong Yongsheng2 Wu Yan2 Xion Yiwei2 Jiang Sufei2(1 Fisheries College,Nanjing Agriculture University,Wuxi,214081,China;2 Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi,214081,China);Studies on Genetic Differences between Male and Female Jian Carp by AFLP Analysis[J];Anhui Agricultural Science Bulletin;2007-12
3 ZHOU Xing-wen et al(Yulin Normal University,Yulin,Guangxi 537000);Research Progress of the Relationship of Tree Peonies[J];Journal of Anhui Agricultural Sciences;2007-02
4 YU Miao1,ZHENG Guo-sheng2,ZHOU Chun-ling1,SUN Yu-lin1,WANG Hong-wei3(1.Qingdao Agriculture University Landscape Aarchitectare and Art College,Qingdao,Shandong 266109,China;2.Qingdao Agriculture University College of Life Science,Qingdao,Shandong 266109,China;3.Horticulture College,Qingdao Agriculture University,Qingdao,Shandong 266109,China);Studies on the Development Strategy for the Tree Peony Core Collection of Shandong Province[J];Northern Horticulture;2008-08
5 Chen Jun-yu (College of Landscape Architecture,Beijing Forestry University,100083, P.R.China);Six Years in International Cultivar Registration for Mei(Prunus mume)——Achievements and Prospects[J];Journal of Beijing Forestry University;2004-S1
6 Liu Qinglin (Department of Landscape Architecture, China Agricultural University, 100094 Beijing,P,R.China);Some Aspects on Research Achievements in Prunus mume in China and Aboad[J];Journal of Beijing Forestry University;2004-S1
7 Li Qingwei (College of Landscape Architecture, Beijing Forestry University,100083,P.R.China);Advances in Mei (Prunus mume) Research[J];Journal of Beijing Forestry University;2004-S1
8 HU Xiao-li~1;ZHOU Chun-jiang~2;YUE Liang-song~3.1 Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,Beijing Forestry University,100083,P.R.China;2 Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing,100101,P.R.China;3 Shandong Tralin Ecology Sci-Tech Co.,Ltd,Gaotang,Shandong,252800,P.R.China.;Molecular identification of Populus tomentosa triploid clones by AFLP[J];Journal of Beijing Forestry University;2006-02
9 LI Qing-wei; CHEN Jun-yu; ZHANG Qi-xiang. National Floriculture Engineering Research Center, College of Landscape Architecture, Beijing Forestry University, 100083, P. R. China;Advances and prospects in the development of scientific and industrial research of Mei (Prunus mume).[J];Journal of Beijing Forestry University;2007-S1
10 WANG Hong-bing1;BO Yu-xin2;LI Xiao-hong3;HU Yong-hong4;LIU Su-qing5.1 College of Landscape Architecture, Beijing Forestry University, 100083, P. R. China;2 Bureau of Landscape Architecture of Nanyang, Henan Province, 473001, P. R. China;3 Bureau of Landscape Architecture of Wuxi, Jiangsu Province, 225400, P. R. China;4 Botanical Gardens of Shanghai, 200231, P. R. China;5 Institute of Landscape Architecture of Baotou, Inner Mongoha, 014010, P. R. China;The comparison of application of Mei flower in Chinese and Japanese classical gardening[J];Journal of Beijing Forestry University;2007-S1
China Proceedings of conference Full-text Database 2 Hits
1 Song Yi-yan Zhou Wu-zhong (Institute of Tourism and Landscape Aarchitecture,Southeast University,Nanjing 210096,China);Plum Blossom and Chinese Tourism Culture[A];[C];2007
2 SONG Chunyan,CHU Ying,CHEN Mingjie,TAN Qi,PAN Yingjie ( Institute of Life Science,Nanjing Agricultural University,Nanjing,210095;Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai,201106; Shanghai Fisheries University, Shanghai,200090);OPTIMIZATION OF AFLP REACTION CONDITIONS BASED ON CEQ GENETIC ANALYSIS SYSTEM[A];[C];2006
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 FANG Cong bing 1,SHENG Bin cheng 2,ZHANG Zhen 2 (1 Department of Horticulture,Anhui Agricultural University,Hefei 230036; 2 Department of Horticulture,Nanjing Agricultural University);Effect of Different Sample-making Methods for SEM on Pollen Morphology of Fruit Mei (Prunus mume Sieb et Zucc)[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;2000-01
3 Mao Hanshu Ma Yan Wang Zhongzhi(Beijing Forestry University);Studies on Numerical Classification of Chinese Mei Flower Cultivars[J];Journal of Beijing Forestry University;1992-04
4 Song Wan; Xu Jiuru. Journal of Beijing Forestry Universtity (2000)22(1)76~80 [Ch,24 ref.] College of Forest Resources and Environment, Beijing For. Univ., 100083, P. R. China.;Progress in application of germplasm identification and fingerprinting of fruit trees.[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;2000-01
5 Zhou Chunling; Dai Silan. College of Landscape Architecture, Beijing For. Univ., 100083, P. R. China.;AFLP analysis of some Dendranthema spp.[J];Journal of Beijing Forestry University;2002-Z1
6 Liu Qinglin(College of Landscape Architecture,BFU 100083);Discussion on the Origin and Cultivar Evolution of Mei[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1996-02
7 Zhang Junwei Bao Manzhu Chen Longqing (Department of Forestry, Huazhong Agricultural University, Wuhan,430070,P.R.China);Analysis of Prunus mume, P.persica, P.salicina, P.armeniaca, P.subhirtella and P.lannesiana by RAPD[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1998-02
8 Chen Junyu (College of Landscape Architecture, Beijing For. Univ.,100083, P.R. China);The New Revised System of Classification for Chinese Mei Cultivars[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1999-02
9 Chen Xianggao Fang Weimin (Department of Horticulture,Nanjing Agricultural University,210095,P.R.China) Wang Shishan Wang Baogen Zhang Siping (Sun Yat sen Mausoleam Administrative Office,Nanjing,210014,P.R.China);Study on Phenology and Display Season Extension of Mei Flower[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1999-02
10 Liu Qinglin Department of Ornamental Horticulture, China Agricultural University, Beijing 100094,P.R.China Chen Junyu College of Landscape Architecture, Beijing Forestry University, Beijing 100083,P.R.China;Effects of Pollen Mentor, Growth Regulator and Embryo Culture on Interspecific Hybridization of Prunus mume[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1999-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved