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《Journal of Beijing Forestry University》 2003-03
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DNA template preparation and technology system building for AFLP analysis of Prunus mume.

Ming Jun;Zhang Qixiang;Yan Xiaolan;Qiu Lijuan. Department of Horticulture, Hubei Agricultural College, Jingzhou, 434025, P. R. China.  
The improved method of SDS DNA isolation is used to extract total DNA from tender or old leaves of \%Prunus mume\% in this study. High quality total DNA from plant was isolated in less than 3 hours. The values of OD 260 / OD 280 of each sample were between 1 7 and 1 9 Strips in agarose electrophoresis were clear and less decomposed. DNA samples with high purification and high yield rate could be gained by this method. It is much more simplified than former methods at many steps. The procedure of the method is easy, fast and effective, and is good to extract genomic DNA of \%Prunus mume\%. These genomic DNA samples are intact without contaminating nucleases or inhibitor. The DNA isolated with this method could be completely digested by restriction enzyme of Mse Ⅰ and EcoR Ⅰ, and good enough for use in following applications including PCR and analysis of AFLP. The systems suitable for silver stained AFLP analysis were established and capable of producing sharp fingerprint patterns.
【Fund】: 湖北省自然科学基金资助项目 ( 2 0 0 1ABB118)
【CateGory Index】: S685.1
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