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《Journal of Beijing Forestry University》 2004-01
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Callus induction and plant regeneration from seeds of Festuca arundinacea

Wang Cheng; Li Qing; Xin Yan. School of Landscape Architectur e, Beijing For. Univ.,100083, P.R.China.  
In this paper, two species of tall fescue seed were cultivated on the MS medium supplemented with different kinds of hormones and nutrients. The effect of 2,4--D,KT, 6--BA, NAA,IAA,lactalbumin hadroly satz and culture c onditions for inducing callus from Festuca arundinacea seeds were described . The frequency of callus induction was different when the same kind of Festuca arundinacea seeds was cultivated in MS medium supplemented w ith different types of and different concentrations of hormones. It was shown that 2,4--D decided the frequency of callus induced from t all fescue seeds, while KT, 6-- BA,NAA,IAA had no significant effect on the callus. The optimum callus in ducing medium of Festuca arundinacea seeds was selected from these kinds of media,and the best one was MS + 6 mg/L 2,4--D + 600 mg/L l actalbumin hadrolysatz. The authors successfully regenerate tall fescue plant by means of cultivating tall fes cue callus on the MS medium supplemented with 2,4--D and KT,a nd th e best plant regeneration medium of tall fescue was MS + 6 mg/L 2,4--D +0.2 mg/L KT. A preliminary plant regeneration system of Festuca ar undinacea was established on the MS medium supplemented with appropriate hormones and nutrients by means of inducing callus and regenerating plants.
【Fund】: 国家科技部转基因植物研究与产业化开发专项计划资助
【CateGory Index】: S688.4
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