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《Journal of Beijing Forestry University》 2004-S1
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Pollen Cryopreservation of Prunus mume

Liu Yan Zhang Yali (College of Landscape Architecture,Beijing Forestry University,100083,P.R. China)  
Pollen cryopreservation of 26 cultivars from different Mei forms was carried out. Pollen germination rate and hybridization of fresh pollen and post-freezing pollen were tested. The effect of different storage methods(5,-20 and -196℃)on pollen germination rate were compared. Results indicate that the pollen of 23 Mei cultivars were preserved by cryopreservation successfully. After storage in liquid nitrigon, the pollen germination rate rises or reduces. Pollen germination rate of 5 cultivars still keep at 34% to 73% after 1 year of storage in liquid nitrigon. The thawing pollen still has good pollination ability, and fruitage rate is higher than that of fresh pollen. After 9 months of storage, the germination ability of pollen storage at 5℃lost and decreased sharply at -20℃, but keep high germination rate at liquid nitrigon. So keeping pollen by cryopreservation is an ideal method for Mei pollen storage at present.
【Key Words】： Prunus mume pollen cryopreservation storage
【CateGory Index】： S685.17
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