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《Journal of Beijing Forestry University》 2006-S2
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Ion compartmentation in leaf cells of Populus euphratica and P.tomentosa under salt stress

DAI Song-xiang~1; CHEN Shao-liang~1; Eberhard Fritz~2;Andrea Olbrich~2; Christine Kettner~2;Andrea Polle~2;Aloys Hüttermann~2. 1 College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083, P. R. China;2 Institut für Forstbotanik, Georg-August-Universitat Gttingen, D-37077, Germany.  
The authors used X-ray microanalysis to investigate ion compartmentation (Na~+, Cl~-and K~+) in leaf cells of a salt_tolerant species, Populus euphratica and a salt_sensitive species, P. tomentosa(treated by salt for 20 days). Regenerated plants were used in this experiment and following types of leaf cells were examined: 1) leaf vein cells—including xylem vessels in the bundle, parenchyma cells in the bundle and around the bundle; 2) mesophyll cells—including palisade and spongy mesophyll cells; 3) epidermal cells—including adaxial and abaxial epidermis. Results showed that there was genotypic difference in subcellular ion compartmentation under salt stress. Compared with P. tomentosa, P. euphratica exhibited a greater ability to exclude Na~+ to the apoplast, furthermore, P. euphratica had a higher capacity to compartmentize Na~+ into vacuoles. Control plants of P. euphratica retained higher levels of Cl~-in all cell compartments (chloroplast, cell wall and vacuole), as compared to the control of P. tomentosa plants. However, stressed P. euphratica remained lower levels of Cl~-than salinised P. tomentosa in all types of leaf cells, this was the result of root-to-shoot salt transport restriction in P. euphratica under saline conditions. With regard to K~+, salinity reduced K~+ level in chloroplast, vacuole and cell wall of all types of leaf cells in P. euphratica with a few exceptions. Unlikely, K~+ levels in the apoplast of P. tomentosa were lowered by salinity, but in vacuole, K~+ increased in response to salt stress. This was presumably resulted from the redistribution of K~+ in cell compartments: K~+ fluxed from the cell wall into the vacuole, which was favorable to balance the negative charges caused by vacuolar Cl~-compartmentation.
【Fund】: 国家自然科学基金重点项目(30430430);; 德国洪堡基金;; 德国DFG基金;; 全国高校优秀博士学位论文作者专项基金(200152);; 高等学校优秀青年教师教学科研奖励计划(2002-323)资助
【CateGory Index】: S792.11
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