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《Journal of Beijing Forestry University》 2007-05
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Effects of genotype on in vitro regeneration from the leaves of Populus tomentosa Carr

YAO Na;ZHANG Zhi-yi;AN Xin-min;WANG Dong-mei;TAO Feng-jie.Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,Beijing Forestry University,100083,P.R.China.  
The leaves of five genotypes(TC121,TC152,TC332,TC510 and TC970) in Populus tomentosa Carr.were used as explants to study the differences of in vitro regeneration competences among genotypes.Single factor and orthogonal experiment designs were used to establish and optimize the leaf regeneration system of P.tomentosa.The results showed that the regeneration competences were affected by genotypes,and there were obvious differences among genotypes in regeneration rates.The regeneration rate of TC152 reached 97.0% on the medium of MS + 6-BA 1.0 mg/L+ NAA 0.05 mg/L + sucrose 30 g/L + agar 6 g/L.The regeneration rate of TC970 was 94.7% on the medium of MS + 6-BA 0.5 mg/L + NAA 0.05 mg/L + sucrose 30 g/L + agar 6 g/L.After medium optimization,the regeneration rate of TC332 had been improved to 72.3%.However,the regeneration rates of TC510 and TC121 remained below 50.0%.
【Fund】: “十一五”国家科技支撑计划课题(2006BAD01A15)
【CateGory Index】: S792.11
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