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《Journal of Beijing Forestry University》 2010-03
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Biological characteristics of Burkholderia sp.caf324 isolated from Mimosa invisa

PENG Yu-hong1;JIAO Ru-zhen1;MOU Xin-tao2.1 Research Institute of Forestry,Chinese Academy of Forestry,Beijing,100091,P.R.China;2 Forestry and Agricultural Biotechnology Institute,University of Pretoria,0002,South Africa.  
Isolated from root nodules of Mimosa invisa Mart.ex Colla in the Jianfengling National Nature Reserve,Hainan Province,a representative strain,caf324,was analyzed by 16S rDNA sequencing,physical and chemical properties test.The 16S rDNA sequence was compared with related rhizobia and an unrooted phylogenetic tree was produced.The results show that caf324 is clustered together with β-rhizobia of Burkholderia mimosarum PAS44(AY752958),with a similarity of 99.7%.Biological characteristics of this caf324 strain were tested,showing the following results:catalase and oxidase were positive,indole was not found,gelatin was not hydrolysed and glucose was unable to generate acid through fermentation.It can endure 2% NaCl and grow in media with pH of 4-11,under the temperatures up to 45℃.
【Fund】: 国家科技基础条件平台项目(2005DKA21207);; “十一五”国家科技支撑计划项目(2006BAD07A13-3)
【CateGory Index】: S154.3
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