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《Journal of Beijing Forestry University》 2010-05
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Construction of multi-gene expression vector and tobacco transformation based on Cre-LoxP recombination system

JIA Xiang-nan1;LI Wei1;SHEN Jun-ling1;OUYANG Kun-xi1;CHEN Xiao-yang1,2 1 National Engineering Laboratory of Forest Tree Breeding,Beijing Forestry University,100083,P.R.China;2 College of Forestry,South China Agricultural University,Guangzhou,510642,P.R.China.  
Using a system of multi-gene plant expression vector based on a set of Cre-LoxP recombination system,the BtCryIAc,BADH,pttGA20ox and rolB genes were inserted into the plant expression vector pYL1305,which was named pYL1305BBGR.The tabacco was transformed by leaf disc method mediated by Agrobacterium tumefaciens.Tested by PCR and Southern blot,the multi genes have been successfully transferred into the genome of tobacco.The semi-quantitative RT-PCR analysis showed that all the 4 genes were expressed normally.
【Fund】: 教育部科学技术研究重点项目(01028)
【CateGory Index】: Q943.2
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