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《JOURNAL OF BEIJING FORESTRY UNIVERSITY》 1999-02
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A Preliminary Research on the Relationship of Prunus mume Cultivars and Its Close Relatives by Using RAPD Assay

Liu Qinglin (Department of Ornamental Horticulture, China Agricultural University, Beijing, 100094, P.R. China) Chen Junyu (College of Landscape Architecture, Beijing For. Univ., 100083, P.R. China)  
The leaf DNA of 28 cultivars of 17 forms of Prunus mume, 8 interspecific hybrids and 8 closely related species were analyzed through RAPD with 3 artificial primers. The RAPD results show that P. mume is closely related with P. armeniaca, then with P. salicina, but far away from P. davidiana and P. tomentosa;the interspecific hybrid of P. tomentosa × P. mume is different from its parents. The effect of mixed primer, templet and reaction volume on appearance and repeatability of polymorphism DNA, and its ability, probability were discussed at the end of the paper.
【Key Words】： Prunus mume relationship relative RAPD
【CateGory Index】： S685.170.21
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