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《Labeled Immunoassays and Clinical Medicine》 2002-01
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Establishment and Primary Application of Interleukin-4 Radioimmunoassay

CHEN Su-juan, DONG Xiao-jun, SUN Xiao-li, et al. (Centre of Science and Technique Development, General Hospital of PLA, Beijing 100853, China)  
The IL-4 antibody was botained by immunizing rabbits with recombinant IL-4.The IL-4 was iodinated with Iodogen methods and purified by the Sephadex G-50 chromatograph column.Radioimmunoassay for IL-4 was first established domestically. The determin range of IL-4 was 0.3-24.3ng/mL.The intra-assay coefficient of variatiors was less than 8%, the inter-assay cofficient of voriatiors was less than 15%. The level of IL-4 in normal peoples was 0.78±0.33ng/mL(n=166).There were no differences in serum IL-4 level between normal peoples and liver cancer patiects who were positive in serum alpha-fetoprotein ( P 0.05).Whereas, the serum IL-4 level in primary hyperthyroidism was significantly higher than that in mormal peoples ( P 0.01).
【CateGory Index】: R446.6
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 HOU Wei,QIAN Dongmeng, YAN Zhiyong, et al\ Department of Microbiology, Qingdao University Medical College, Qingdao 266021\;;CONSTRUCTION OF RECOMBINANT CLONE OF SITE-SPECIFIC GENE MUTANT OF HUMAN INTERLEUKIN-2[J];Acta Acadimae Medicinae Qingdao;2003-01
2 GUO Jian, SONG Da-lin, WANG Yan-ming (Laboratory of Endocrinology, Qingdao Hiser Medical Group, Qingdao 266033, China);;ESTABLISHMENT OF MODEL IN DIABETES MELLITUS COMPLICATED WITH INFECTION AND CHANGES OF TUMOR NECROSIS FACTOR α IN RATS[J];Acta Acadimae Qingdao Umvesitatis;2005-01
3 MENG LIN,LIU MING-JUN,WANG BIN, et al(Department of Microbiology,Qingdao University Medical College,Qingdao 266021,China);CLONING AND PROKARYOTIC EXPRESSION AND ACTIVITY OF ~(88)Arg IL-2[J];Acta Academiae Medicinae Qingdao Universitatis;2006-04
4 CHEN Li-Long,ZHU Xiao-Tong,JIANG Qing-Yan **,GAO Ping,SHU Gang,FU Wei-LongCollege of Animal Science, South China Agricultural University, Guangzhou 510642, China;Effects of synthetic bursin on proliferation of chicken splenic lymphocytes and expression of IL-2 and IL-6[J];Acta Zoologica Sinica;2006-01
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6 CHEN Cui-li~1,SHI Ding-ji~2(1.College of Arts and Science of Beijing Union University,Beijing 100083,China;2.China Institute of Botany,The Chinese Academy of Science, Beijing 100093,China);Construction of Anabaena Transferred the Gene Encoding the Human Granulocyte Colony-stimulating Factor[J];Journal of Beijing Union University;2005-03
7 NIU Ying-peng(College of Physical Education Science of Henan University,Kaifeng 475001,Henan China);The Effect of EGb on the Activities of CK,LDH and IL-4 in Blood of Rats[J];Journal of Beijing Sport University;2006-10
8 Yao Huiqin,Wang Yang,Zheng Jie,et al (Beijing Tongren Hospital,Beijing 100730);Study on levels of IL-8 in serum and urine of patients with acute urinary tract infection[J];Beijing Medical Journal;2001-01
9 LI Yan,DU Jin-kai,LI Ru-hong,et al (Affiliated Hospital of Chengde Medical College, Hebei Chengde 067000,China);STUDY ON THE EXPRESSIVE CHANGES OF BFGF IN HYPERTENSIVE RAT’S CARDIAC MUSCLE CELLS DURING DIFFERENT PHASES[J];Journal of Chengde Medical College;2004-01
10 ZHAO Xiang-yang, LI Yu-hong, XU Qian, et al(266 Hospital of Chinese People's Liberation Army, Hebei Chengde 067000, China);STUDY ON THE EXPRESSION OF IL-6, IL-6R AND APOPTOSIS-ASSOCIATED GENES IN GASTRIC CARCINOMA[J];Journal of Chengde Medical College;2005-03
【Co-references】
Chinese Journal Full-text Database 4 Hits
1 LIU Zhe YANG Ying CHEN Yun PENG Jing Pian  (State Key Laboratory of Reproductive Biology, Institute of Zoology, The Chinese Academy of Sciences, Beijing 100080,China);INFLUENCE OF hrIFN_γ ON RABBIT PREGNANCY[J];Acta Zoologica Sinica;2002-02
2 ZHAHG Jun 1, YU San ke 2 (1.Qinghai Academic of Animal Husbandry and Veterinary Science, Xining,81003; 2.College of Animal Science and Veterinary Medicine, Northwest Sci Tech University of Agriculture and Forestry );Gamma Interferon-mediated Role Against Toxoplasma Gondii and its Mechanism[J];PROGRESS IN VETERINARY MEDICINE;2000-01
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【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 ZHAO Jin1) SI Li-fang1) XU Yong-ping1) LI Wen-xian2) FAN Guang-li1)(1)College of Animal Science and Technology,Northwest Science Technology ofAgriculture and Forestry,Yangling 712100,China; 2)Sanlu Huahuaniu Latex Limited Company,Zhengzhou 450008,China);The Effect of Exogenous IFN-γ on the Expression of IL-4 Immunoreactive Cells in Hypothalamus of Pregnant Rat in Preimplantation[J];Journal of Huazhong Agricultural University(Natural Science Edition);2007-02
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