Establishment and Primary Application of Interleukin-4 Radioimmunoassay
CHEN Su-juan, DONG Xiao-jun, SUN Xiao-li, et al. (Centre of Science and Technique Development, General Hospital of PLA, Beijing 100853, China)
The IL-4 antibody was botained by immunizing rabbits with recombinant IL-4.The IL-4 was iodinated with Iodogen methods and purified by the Sephadex G-50 chromatograph column.Radioimmunoassay for IL-4 was first established domestically. The determin range of IL-4 was 0.3-24.3ng/mL.The intra-assay coefficient of variatiors was less than 8%, the inter-assay cofficient of voriatiors was less than 15%. The level of IL-4 in normal peoples was 0.78±0.33ng/mL(n=166).There were no differences in serum IL-4 level between normal peoples and liver cancer patiects who were positive in serum alpha-fetoprotein ( P 0.05).Whereas, the serum IL-4 level in primary hyperthyroidism was significantly higher than that in mormal peoples ( P 0.01).