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《Labeled Immunoassays and Clinical Medicine》 2004-04
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Clinical Evaluation of Two Methods for Determination of Urinary Microalbumin

SUN Qi, YANG Guo-hua, WANG Heng (Department of Endocrinology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China)  
To evaluate the clinical effect of two methods for determination of urinary microalbumin with chemiluminescence immunoassay(CLIA) and RIA, and to select a method which is simple, quick, accuracy and specificity, 8 hour urine was collected in 85 diabetic patients and 80 health controls, and determined by CLIA and RIA. The precision, sensitivity, specificity and interference were also analyzed in two methods.Results showed that both methods were precise, having a inter-assay CV4% and intra-assay CV6%.Recoveries of two methods were 95.1%-105.9%.It was free from γ globulin.The lowest detectable concentration was 2.5μmol/L in CLIA.The urinary albumin levels were measured by the CLIA methods correlated well with those by RIA method, Y=0.9974X-0.0291, r=0.9902, in 85 diabetic patients and 80 health controls.In conclusion, the two methods for determination of urinary microalbumin were precise, specificity and significantly correlated.However, CLIA was more simple, quick, reliable and without contaminated of radioisotope compared with RIA. The method can be automated which is suitable to be clinically used as a routine laboratory test.
【CateGory Index】: R446.6
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