Antitumor Cytokines Production by Peripheral Blood Mononuclears Cells in Patients with Gastric Carcinoma and Lung Cancer
Bai Jun; Wang Li; Zang Xingxing.et al.(Shanghai Institute of Immunology, Shanghai Second Medical University, Shanghai 200025)
Levels of interleukin-2 (IL-2), interferon-γ(IFN-γ) and tumor necrosis factor (TNF) produced by peripheral blood mononclear cells (PBMC) after incubation with phytohemagglutinin were measured by 3H-TdR incorporation and other biological assays in 72 patients with carcinomas and 10 healthy subjects. The findings showed that:(1) the ability to produce IL-2 and IFN-γ were siguificantly lower in patients with gastric carcinoma, lung cancer or other cancers than in the controls, the levels of IL-2 and IFN-γ in patients being 1/29～1/35 and 1/16～1/19 of the control, respectively;(2) the ability to produce TNF was markedly higher in patients with gastric carcinoma, lung cancer or other cancers than in the controls, the level of TNF in patients was 8～12 times of the controls;(3) after resection of gastric carcinoma,levels of IL-2 and IFN-γ increased, while TNF decreased. Our results suggest that antitumor cytokines such as IL-2, IFN-γ and TNF play an important roles in the relationship between the immune system and cancer and that the measurement of these cytokines were valuable in the diagnosis and therapy of the cancer patients.
【CateGory Index】： R730.21