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《LABELED IMMUNOASSAYS AND CLINICAL MEDICINE》 1999-02
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Study on Immunogold Quantitative Assay for Microalbuminuria

Chu Xuntao1,Lu Shengkai2,Dong Yongqin3et al. (1 Laboratory of Medical Technology,Shanghai Second Medical University,Shanghai 200023; 2 Shanghai Centre for Clinical Laboratory Sciences,Shanghai 200052; 3Department of Medical Laboratory Sciences,Shanghai  
Using gold sol particles as the carrier of immuno-ligands,an immunogold agglutination inhibition assay was establishead to quantify microalbuminuria.In this assay system,the suitable diameters of the gold sol particles were 5070nm.The flocculated sediment of the collidal gold in urine medium was diminished by using borate buffer as the reaction buffer.A final concerntration of 3% of polyethylene glycol 6000 ensured the agglutination test to be fulfilled completely.The linear range of the calibration curve of the method was 40200mg/L,which was suitable for the assay of microalbuminuria.103 clinical urine specimens were determined by this method,and their results were compared with those of rate nephelometry on automated protein analyzer.The correlation of these two methods reached 995%.
【CateGory Index】: R446.6
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【References】
Chinese Journal Full-text Database 1 Hits
1 CAO Danru,ZHANG Jinfeng,LIU Decong,ZHU Qinghui,TAO Yixun. Shanghai Centre for Clinical Laboratory,Shanghai 200126,China;Studies on the semi-quantitative gold immunofiltration assay for rapid detection of microalbuminuria[J];Shanghai Journal of Medical Laboratory Sciences;2004-05
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 YU Wei 1,2 ,LIAO Jian-hui 1 ,GE Cun-wang 1 ,GU Ning 1 ,WU Jian-guo 2(1.National Laboratory of Molecular and Biomolecular Electronics,South east University,Nanjing210096,China;2.Laboratory of Clinical Im munol ogy,Center of Medical Laboratory Science,Jinling Hospital of Nanjing,Nanjing 210002,China);Electrophoresis of bare and proteins-capped gold colloidal nanoparticles[J];Micronanoelectronic Technology;2003-09
2 Wang Hanzhong; Liang Li(Wuhan Institute of Virolog, Academia Sinica, Wuhan 430071)This paper reported that porcine parvovirus (PPV)can been detected from infected and clinical samples using nested PCR. External and internal primers were selected f;PPLICATION OF NESTED PCR ASSAY FOR DETECTION OF PORCINE PARVOVIRUS[J];Chinese Journal of Virology;1996-02
3 ZHAO Jun-long,CHEN Huan-chun,LV Jian-qiang,XIAO Shao-bo,ZHOU Fu-chun (College of Animal Science and Veterinary Medicine,Huazhong Agricultural University,Hubei,WUHAN 430070,China);Study on Gene Immunity of Porcine Parvovirus Structural Protein Genes VP_1 and VP_2[J];Chinese Journal of Virology;2003-01
4 Ma Lin; Wang Yujin; Yang Shuhao; et al.(Zheng Zhou Biocell Biotechnology Institute, Zheng Zhou 450003);Rapid Colloidal Gold Immunochromatographic Assay for HBsAg and Its Application[J];LABELED IMMUNOASSAYS AND CLINICAL MEDICINE;1997-04
5 Li Chenxu, Liu Hongyang, Liu Cheng,Sun Changjie(1 The Central laboratoy,The School of Preventive Medicine;2 The First Clinical College. ; 3 Changchun Hospital of Tuberculosis);Diagnosis of HBsAg by immunochromatography[J];JOURNAL OF NORMAN BETHUNE UNIVERSITY OF MEDICAL SCIENCE;1998-04
6 Lan Xinzhe 1,.2 ,Jin Zhihao 1 ,Zhao Xicheng 2 ,Gou Linfeng 2 (1.Xi ' an Jiaotong University,Xi ' an 710049,China)(2.Xi ' an University of Architecture and Technology,Xi ' an 710055,China);Preparation of Nanogold Colloid by C hemical Reducing with PVP Protection[J];Rare Metal Materials and Engineering;2003-01
7 ZHENG Kui yang 1, DU Wen ping 1, LIU Yi sheng 1, ZHENG Xia 1, FU Lin lin 1, WU Zhong xing 2 (1. Xuzhou Medical College, Xuzhou, Jiangsu 221002, China; 2.Institute of Parasitic Diseases of Jiangsu Province, Wuxi, Jiangsu 214064,China);Establishment and Primary Application of Fast Dot-immunogold Staining for Detection of Antibodies to Cysticercosis[J];Endemic Diseases Bulletin (China);2002-02
8 ZHOU Jian zhang,WU Ling ling,DONG Li qin,LIN Zhong yu, YAN Jia wei,DONG Ping,BAO Yue,LIN Zhong hua (State Key Lab. for Phys. Chem. of the Solid Surface, Dept. of Chem., Inst. of Phys. Chem., Xiamen Univ., Xiamen 361005, China);Immobilization of Long-Strand DNA onto Gold Surface and Electrochemical Labeling of Hybridization of DNA[J];Electrochemistry;2001-03
9 LU Jian-qiang, CHEN Huan-chun, ZHAO Jun-long (Animal Husband and Veterinary College, Huazhong Agricultural Universit y, Wuhan, 430070,China);Advancement on the Research of Porcine Parvovirus Vaccine[J];Progress In Veterinary Medicine;2002-01
10 HU Dong wei 1 LANG Xiao lan 2 YUAN Ya ling 3 WU Yun feng 1 (Dept.Plant Protection,Northwestern Agricultural University,Yangling,Shanxi 712100,China.) (Teaching College,Xi'an University,Xi'an 710061,China.) (The 3rd Laboratory,Xi'an Plant Garden,Xi'a;Immuno gold identification and detection on pathogens of tulip breaking syndrome[J];JOURNAL OF CHINESE ELECTRON MICROSCOPY SOCIETY;1999-02
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 CAO Danru, ZHENG Zuoya, ZHANG Jinfeng, LIU Decong, ZHU Qinghui, TAO Yixun. Shanghai Centre for Clinical Laboratory, Shanghai 200126, China;Studies on the semi-quantitative gold immunofiltration assay for rapid detection of alpha-fetoprotein[J];Shanghai Journal of Medical Laboratory Sciences;2005-03
2 CAO Danru1,CHEN Yue1,LIU Decong2,ZHU Qinghui2,TAO Yixun1.(1.Shanghai Centre for Clinical Laboratory,Shanghai 200126,China;2.Department of Clinical Laboratory,the People′s Hospital of Shanghai Putuo District,Shanghai 200060,China);Development of a semi-quantitative gold immunofiltration assay for rapid detection of serum human chorionic gonadotropin[J];Laboratory Medicine;2008-01
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