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《BEIJING BIOMEDICAL ENGINEERING》 1999-01
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Study of Fine Structures of the Natural Surface of Free Radical Treated Red Blood Cells with Atomic Force Microscopy

Huang Yimin,Li Jia,Liu Danjing,Yu Xin,Zhao Hui (Beijing Institute of Heart Lung & Blood Vessel Diseases,Beijing\ 100029) Zhang Pingcheng,Bai ChunLi (STM Lab.,Institute of Chemistry,Chinese Academy of Sciences,Beijing\ 100080) The subject is supp  
Objective\ To observe the effects of free radicals(Fr),ligustrum lucidum ait (LLA)+Fr,and H 2O 2 on the natural molecular morphology of red blood cell membrane surface directly.Methods\ Using an atomic force microscopy(AFM) with 0 1 nanometer resolution to scann the glutaraldehyde fixed membrane surface of RBCs,which have been treated by Fr,LLA+Fr,H 2O 2 respectively;and on nanometer range,in room temperature and air conditions;and to compare with SEM images results of.Results\ After Fr treatment, visual polymers with various sizes,ranging from tens to hundred nanometers,aopear at protein particles of RBC membrane surface the membrane surface roughness being at nanometer level.After recovering for 3h,these particle changes decreased partly.LLA+Fr or H 2O 2 treatment just resulted in smaller size polymers formation,and increase of seams as well between particles.Conclusion\ (1)AFM offers relatively quick and easy specimen preparations than SEM,without requirements to dehydrate in graded ethanol and dry at critical point,and no sputter coating.It also offers direct observing living or fixed biological specimen's surface to get molecular topography at room temperature and air conditions;(2)AFM can clearly distinguish the molecular characteristic structures of RBC membrane surface after treatment with Fr,LLA+Fr or H 2O 2.That is,the surface change of the RBC membrane,treated by LLA+Fr and H 2O 2,were less then changes those,treated with Fr at molecular topographical level;(3)AFM can do 3 dimensional quantitatie analysis for the observed RBC molecular morphology at membrane surface.
【Fund】: 国家自然科学基金 北京市自然科学基金
【CateGory Index】: R331.141,Q-336
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