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《Beijing Medical Journal》 2002-06
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Effect of MIP-1α on the proliferation of synovial fibroblasts from RA patients

Wang Bin,Tang Fulin,Li Jun,et al (Department of Rheumatology and Clinical Immunology,Peking Union Medical College Hospital,Beijing 100730)  
Objective To compare the difference of macrophage inflammatory protein 1α (MIP 1α),MIP 1β and regulated on activation,normal T cell expressed and secreted (RANTES) production by human synoviocytes in rheumatoid arthritis (RA),osteoarthritis (OA) and post traumatic (PT) patients and to investigate whether the MIP 1α can induce the proliferation of synovial fibroblasts from RA patients.Methods To collect the synovial tissue from the RA,OA and PT patients,synoviocytes were obtained by digesting synovial tissue with collagenase.Fibroblasts derived from explant culture of tissue fragment.MIP 1α,MIP 1β and RANTES were detected by ELISA kit.Fibroblast proliferation was assayed by MTT method.Results Synoviocytes from RA,OA and PT patients did not spontaneously produce MIP 1α,MIP 1β and RANTES.When stimulated with lipolysaccharide (LPS,5μg/ml) and interleukin 1α (rhIL 1α,50U/ml),the synoviocytes from RA patients produced much more contents of MIP 1α and RANTES than that of OA and PT patients.There is no difference in contents of MIP 1β secreted by synoviocytes from the three types of patients.MIP 1α (2~50ng/ml) could increase the proliferation of synovial fibroblasts from RA patients in a dose dependent manner.Conclusions Synoviocytes from RA patients produce higher contents of MIP 1α and RANTES than that of OA and PT patients.MIP 1α could enhance the proliferation of synovial fibroblasts from RA patients.These results suggested that the pathogenesis of RA may be associated with MIP 1α.
【CateGory Index】: R593.22
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