Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Beijing Medical Journal》 2008-03
Add to Favorite Get Latest Update

The killing effect of human Macrophage induced by Newcastle Disease Virus to Hep-2 tumor cells in Vitro

ZHANG Ping, PENG Jun (Department of ENT Section Affiliated Hospital of North China Coal Medical College, Tangshan 063000)  
Objective To study the antitumor activity of human Macrophage(Mφ) induced by Newcastle Disease Virus (NDV) and UV-inactivated NDV (NDV-UV) in vitro. Methods Peripheral blood mononuclear cells (PBMC) were purified via Ficoll-Paque from human peripheral blood and human Mφ were obtained with plastic-adhering method. The human Mφ stimulated with NDV and NDV-UV for 16 hours were acting as effect cells, and Hep-2 tumor cells were acting as target .The tumor-cytotoxic effects of human Mφwere detected by MTT method and further compared between induction of NDV and NDV-UV. Results The tumoricidal activity of human Mφ induced by NDV and NDV-UV was higher than non-induced group (P﹤0.01). The tumor-cytotoxic effects of human Mφ induced by NDV was stronger than NDV-UV group (P﹤0.01). Conclusions Both NDV and NDV-UV can promote tumoricidal activity of human Mφ in vitro, and the tumoricidal activities of NDV-stimulated human Mφ seem to be higher than that of NDV-UV stimulated ones.
【CateGory Index】: R739.65
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 SHEN Yong-zhou et al (Anhui Agricultural University,Hefei,Anhui 230036);Isolation and Identification of Newcastle Disease Virus from Pig[J];Journal of Anhui Agricultural Sciences;2006-19
2 YUAN Zhang et al(Faculty of Animal and Technology,Sichuan Agricultural University,Yaan,Sichuan 625014);Research Progress in the Pseudorabies Virus-Vector[J];Journal of Anhui Agricultural Sciences;2007-06
3 WANG Yan et al (Zhengzhou College of Animal Husbandry and Veterinary Medicine,Zhengzhou,Henan 450011);Cloning and Sequence Analysis of gE Gene of Infectious Laryngotracheitis Virus Isolated from HN Changge Strain[J];Journal of Anhui Agricultural Sciences;2007-28
4 LIAO Lie-ru et al(College of Animal Science and Technology,Northwest A & F University,Yangling,Shaanxi 712100);Isolation and Identification of Canine Coronavirus[J];Journal of Anhui Agricultural Sciences;2007-30
5 SUN Rui (Zhengzhou Railway Vocational and Technical College,Zhengzhou,Henan 450052);Comparative Study of Histopathology on Canine Distemper and Canine Parvovirus Disease[J];Journal of Anhui Agricultural Sciences;2008-13
6 HANG Bo-lin et al (College of Animal Science,Henan Institute of Science and Technology,Xinxiang,Henan 453003);Establishment of PCR Method for Detecting Porcine Circovirus 2[J];Journal of Anhui Agricultural Sciences;2008-16
7 TANG Chang-ping(Institute of Supervising Animal Slaughtering of Hefei,Hefei,Anhui 230001);Diagnosis and Prevention of Suspected Swine Vesicular Disease[J];Journal of Anhui Agricultural Sciences;2008-20
8 ZHANG Dun-lin et al (College of Chemical Science and Engineering,Yunnan University,Kunming,Yunnan 650091);Research on the Effects of Polysaccharides from Zhiqi Fungal Substance on CEF Proliferation and Resisting NDV Infection[J];Journal of Anhui Agricultural Sciences;2008-20
9 ZHAN Song-he et al (Anhui Center for Animal Disease Control and Prevention, Hefei, Anhui 230022);Comparative Test on the Immune Effects on Piglets of Inactivated PRRS Vaccines (NVDC-JXA1 Strain) from 3 Manufacturers[J];Journal of Anhui Agricultural Sciences;2008-21
10 MI Zhi Qiang1, JIN Ning Yi1, SUN Ying Chun1, LI Xiao1, LIAN Hai1, LI Jie2, GUAN Guo Fang3 1.Key Genetic Engineering Laboratory of PLA,Quartermaster University of PLA, Changchun,Jilin,130062, P.R.China 2.Hematology Tumor Department, The First Hospital of Jilin University, Changchun,Jilin,130021, P.R.China 3.Otolaryngological Department,The Second Hospital of Jilin University, Changchun,Jilin,130041, P.R.China;Antitumor Research on Mouse Melanoma with Combined Application of Newcastle Disease Virus and Its HN Gene[J];Chinese Journal of Cancer;2004-08
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 HAN Shao-rong, WANG Sen-ming, ZHANG Ji-ren, ZHANG Jian, CAO Man-ming, LI Xiao-ping (Department of Oncology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China; Department of Hepatobiliary Surgery, Nanfang Hospital, First Militar;Generating dendritic cells by mobilizing human peripheral hematopoietic precursor cells[J];Journal of First Military Medical University;2000-04
2 Zhu Huifen; Zhang Yue; Wang Xiaolin; Shao Jingfang ;Shen Guanxin (Laboratory of Immunology, Reseach Center of Experinentat Medicine,Tongji Medical University,Wuhan 430030);Determination of IL-2 and Cytotoxicity of Killer Cells in MTT Colorimetry[J];IMMUNOLOGICAL JOURNAL;1994-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved