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《Beijing Medical Journal》 2012-03
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Application analysis on detection of four serum indicators with a domestic chemiluminescence reagent in liver fibrosis diagnosis

JIANG Fei-fei, WANG Wen-jing, ZHAO Xiu-ying, et al (Department of Clinical Laboratory, Beijing You'an Hospital, Capital Medical University, Beijing 100069)  
Objective To investigate diagnostic value of liver fibrosis for detecting the serum indicators of hyaluronic acid(HA), laminin(LN), procollagen Ⅲ N-terminal peptide (PⅢNP) and collagen type Ⅳ(CⅣ)with domestic chemical luminescence (CL) reagent. Methods Serum indicators of HA, LN, PⅢNP and CⅣwere detected with CL reagent in 123 cases of chronic hepatitis patients by pathological diagnosis(case group) and 30 healthy control(control group). Case group were divided into S0~S4 stages and diagnostic value was evaluated. Results It showed that the HA、LN、CIV in the case group were significantly higher than in control group[(4.47±0.86) vs. (2.02±0.23), P = 0.000;(4.15±0.81) vs. (3.57±0.67), P = 0.002; (3.49±0.89) vs. (2.15±0.81), P = 0.000]. All of the four serum indicators showed control group S0, S1 S2, S2 S3, HA and CⅣ dropped, LN and PⅢNP rose in S4 phage. There was statistically significance of four serum indicators in different liver fibrosis stages (P 0.01). It showed that there was significant difference between S0 and S2 group, S1 and S2 group (P 0.05). The other 3 indicators except HA were significantly correlated with fibrosis stage (P 0.01). Areas under receiver operating characteristic(ROC)curve (AUC) for diagnosis of liver fibrosis were CⅣ(0.790)HA(0.775) LN(0.725)PⅢNP(0.644), successively. Multiple serum indicators showed high sensitivity and accuracy of HA , three combination of HA+LN+CⅣand four indicators combination in diagnosis of liver fibrosis. Comprehensive diagnosis efficiency was CⅣLNPⅢNP. Positive likelihood ratio of single to combination detection was 1~3. Conclusions The four indicators have certain value for diagnosis of liver fibrosis, however, it is limited in diagnosis of early liver fibrosis. It has prominent significance in fibrosis S2 stage. With aggravation of liver fibrosis, HA and CⅣ reversely decline. It may be related with decomposition and reduction of extracellular matrix. It is suitable for application of multi-indicator combination to exclude liver fibrosis. Positive likelihood ratio of all single and combination detection to diagnose liver fibrosis is much lower. It indicates limited diagnosis value and can't substitute liver biopsy.
【Fund】: 国家自然科学基金(30973388);; 吴阶平医学基金会肝病实验诊断研究基金(LDWMF-SY-2011A002)
【CateGory Index】: R575.2
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【Citations】
Chinese Journal Full-text Database 4 Hits
1 LUO Ruihong, YANG Shaoji, XIE Junqiang, et al. Department of Infectious Diseases, Third Affiliated Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou 510630, China;Diagnostic value of five serum markers for liver fibrosis[J];Chinese Journal of Hepatology;2001-03
2 LI Chengzhong, WAN Mobin, ZENG Minde, et al. Changhai Hospital, Second Military Medical University, Shanghai 200433, China [;A preliminary study of the combination of noninvasive parameters in the diagnosis of liver fibrosis[J];Chinese Journal of Hepatology;2001-05
3 LU Lungen, ZENG Minde, WAN Mobin, et al. Zha Bei Qu Central Hospital, Shanghai 200070;A preliminary study on the grading and staging of hepatic fibrosis, and its relations with noninvasive diagnostic parameters[J];Chinese Hepatology;2000-04
4 XUE YONG-JIE1,YANG GUO-RONG1,ZHANG WEN-JIE2,et al.Department of Pathology,the First Hospital of PLA(Lanzhou Gasu,730030)China;Diagnostic value of detection of serum HA、LN、PCⅢ、CⅣ for liver fibrosis[J];Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases;2010-03
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 NING Yu,ZENG Jin.Shenzhen Longzhu Hospital,Guangdong,Shenzhen 518055,China;Clinical observation on high-dose Herbs for Promoting Blood Circulation to Remove Blood Stasis in treatment of liver fibrositis[J];Chinese Journal of Clinical Rational Drug Use;2009-04
2 XIE Yong-zhong,XIA Xia-rong,RAO Bin.The Second People's Hospital Shangrao csity,Jiangxi,Shangrao 334000,China;The influence of Shengqishiyiwei granules combined with recombinant interferon α_2b on WBC AND PLT count in patients with chronic virus hepatitis B[J];Chinese Journal of Clinical Rational Drug Use;2009-04
3 YU Zhan-guo,LIAO Bu.Hospital Dongguan City,Guangdong,Dongguan 523400,China;Observation on Shuanghuqinggan granule in the treatment of liver fibrosis in patients with chronic hepatitis B[J];Chinese Journal of Clinical Rational Drug Use;2009-06
4 LI Wei-chang,YANG Jun.Hebei TCM Hospital for Hepatopathy,Hebei,Shijiazhuang 050800,China;Observation on the effectiveness of Tuihuang Jiang'an decoction in treating Ⅲ~Ⅳ hepatic encephalopathy[J];Chinese Journal of Clinical Rational Drug Use;2009-07
5 GONG Da-lun.The Center Hospital of Penglai Town,Sichuan,Daying 629300,China;The observation of effect of adefovir dipivoxil treated with chronic hepatitis B[J];Chinese Journal of Clinical Rational Drug Use;2009-21
6 WU Chao-yu,JIA De-xing,WANG Wan-peng,et al.Department of Infectious Disease,Weifang Medical College,Shandong,Weifang 261042,China;The relationship and clinical significance in IL-6 of serum and T-lymphocyte subsets,HBV DNA and liver function in patients with HBV[J];Chinese Journal of Clinical Rational Drug Use;2010-02
7 LIAO Zhi-shun,CHEN Yu-hua,LI Yan.The Second Hospital of Nongken,Zhanjiang,Guangdong 524272,China;The effect observation of xueshantong combined with reduced glutathione reduced glutathione in cirrhosis[J];Chinese Journal of Clinical Rational Drug Use;2011-07
8 LI Chen,LIU Qing.Department of Infectious Disease,Fujian Medical University Union Hospital,Fuzhou,Fujian 350001,China;Etiological study on 114 cases elderly patients with hepatitis-markers-negative liver dysfunction[J];Chinese Journal of Clinical Rational Drug Use;2011-21
9 LIU Qiu-li*,JIA De-xing,WANG Wan-peng,et al.*Internal Medicine Department of Weifang Medical College,Weifang,Shandong 261053,China;Detection on serum thrombopoietin levels in patients with Hepatitis B cirrhosis and it′s clinic significance[J];Chinese Journal of Clinical Rational Drug Use;2012-02
10 CHEN Zhao-xiu1,XU Jian-min2,WANG Xiao-cui1,LI Ai-guo1,TIAN Xiang-ping2,YANG Huan-dong3 The Wei Fang Yi Du Center Hospital,1Blood Transfusion Department,2Gestroenterology,3General Surgery Department(Shandong Qingzhou,262500);Changes and significance of immune function in hepatitis patients with liver cirrhosis and ascites before and after fresh frozen plasma transfusion[J];Chinese Journal of Current Advances in General Surgery;2011-11
China Proceedings of conference Full-text Database 10 Hits
1 Hospital of Jiangxi Medical College of Gastroenterology Xiongming Fang,Han Yubin~1,Hu Jia~1, Wang Liping,Xie Hui group~ 2,Xu Yun~ 2,Amateur Training~2,key wind Mei,Yang Satellite.;Softening Hard removing blood stasis of schistosomiasis cirrhosis Affiliated[A];[C];2010
2 YIN Yan-yao,ZONG Ya-li,LIN Yun-hua Ninth Hospital of Nan Chang,Nanchang 330002,China;Clinical research of Chinese herbal retention enema by Total Colonic Dialysis in Treating Hepatic encephalopathy[A];[C];2010
3 Xiao yingqun;To study diagnosing significance of pathology in occult hepatitis B[A];[C];2010
4 LOU Jian-guo,FU Zhi-min,GON Qi-ming,et al.The Tradition Medical Hospital of Jinhua City;Clinical study of sequential treatment with Wenyang Huayu Decoction and lamivudine on patients with chronic hepatitis B[A];[C];2011
5 A yongjun Li Li Li Xiaoyan,Department of Hepato-Bilio Surgery,the Second Hospital Affiliated to Kunming Medical College,Kunming 650101,China;Application of Artificial Liver Support System in Liver Failure of Severe Hepatitis and Liver Transplantation[A];[C];2004
6 YU JianWu Ll XiaoGuang ZHAO YongHua, Department of Infectious Diseases,The Second Affiliated Hospital,Harbin Medical University,Harbin 150086,China;Clinical study of patients with severe hepatitis treated with protein adsorbent recirculating system[A];[C];2005
7 WANG Shuqin YU JianWu lvlingying. Department of Infectious Diseases,The Second Affiliated Hospital,Harbin MedicalUniversity,Harbin 150086,China;Causes and nursing care for the change of blood pressure undergoing plasma exchange[A];[C];2005
8 YU JianWu WANG GuiQiang ZHAO YongHua, Department of Infectious Diseases,The Second Affiliated Hospital,Harbin Medical University,Harbin 150086,China;Prognosis of Patients with fulminant Hepatitis after plasma exchange treatment using MELD scoring system[A];[C];2005
9 LU Zhao-hui ZHANG Qu-xia CHANG Li-Fei, Department Of Infectious Diseases,Anhui Provincial Hospital,Hefei 230001;A clinical assess of artificial liver supporting systems on patients with fatal hepatitis[A];[C];2005
10 YUAN Chun HUANG chang-xing LIAN Jian-qi. The Center of Diagnosis and Treatment for Infectious Diseases of PLA,Tangdu Hospital,Fourth Military Medical University,Xi'an 710038,China;Study on detection of bacterial DNA in ascites of patients with cirrhosis by PCR[A];[C];2005
【Co-references】
Chinese Journal Full-text Database 9 Hits
1 WANG Jun,ZHAO Quan,LU Ying,et al.Dept.of Medical Examination,the 401st Hospital of PLA,Qingdao,Shandong 266071,China;Clinical evaluation on the diagnosis value of LN level in serum for hepatic fibrosis[J];Practical Journal of Medicine & Pharmacy;2011-02
2 ZHANG Ling-rong(Dept of Infectious Diseases,First Clinical Medical College,Shanxi Medical University,Taiyuan 030001,China);Assessment of hepatic fibrosis and liver function indices in 310 patients with hepatitis B[J];Journal of Shanxi Medical University;2009-01
3 ZHANG Yanliang,HONG Dingling,BIAN Weiliang,SHEN Yuying,YAN NingDepartment of Hepatopathy,the First Hospital of Nanjing Medical University,Nanjing 210006,China;Value of FIB-4 for the diagnosis of liver fibrosis in patients with chronic hepatitis B[J];Chinese Journal of Gastroenterology and Hepatology;2009-03
4 Xia Zhu, Hong Tang, Center for Infectious Diseases, West China Hospital, Sichuan University; Division of Infectious Diseases, State Key Laboratory of Biotherapy (Sichuan University), Chengdu 610041, Sichuan Province, China;Application value of Fibroscan in the diagnosis of hepatic fibrosis[J];World Chinese Journal of Digestology;2010-09
5 WEN Dao-lin,WU Shao-qing (Common Bright Hospital of Newly Developed Area of the Brightness of Shenzhen of Guangdong Province,518106,China);Relationship of Liver Function Indexes with the Liver Fibrosis Indexes in Patients with Cirrhosis[J];Guide of China Medicine;2009-07
6 LI Jichang1 DUN Guoliang2 1.Digestion Medicine,Baoji Municipal Central Hospital,Baoji 721008 China;2.Function Department,Baoji Municipal Central Hospital,Baoji 721008,China;Correlation of Liver Pathological Staging with Non-invasive Indexes in Diagnosis of Hepatic Fibrosis[J];China Modern Doctor;2010-12
7 ZHANG Wen-sheng;WANG Bao-en;WANG Tai-ling;OU Xiao-juan;CHEN Yu;LI Qin;WANG Xiao-ming;QIAN Lin-xue;MA Hong;JIA Ji-dong Liver Research Center, Beijing Friendship Hospital, Capital University of Medical Sciences, Beijing 100050, China;Noninvasive assessment of liver fibrosis in chronic hepatitis B using a predictive model[J];Chinese Journal of Hepatology;2006-03
8 ZHAO Zhi-feng JIA Qiu-long(Infectious disease hospital of Xinxiang,Henan 453000,China);The detection of Serum HA,LN,IV C and PⅢNP C of liver fibrosis in chronic hepatitis diagnosis of clinical significance[J];Medical Journal of Chinese People's Health;2012-07
9 Giada Sebastiani,Department of Digestive Diseases,Hepatology and Clinical Nutrition,Dell'Angelo Hospital,30100 Venice and Venetian Institute of Molecular Medicine,35100 Padova,Italy;Non-invasive assessment of liver fibrosis in chronic liver diseases:Implementation in clinical practice and decisional algorithms[J];世界胃肠病学杂志(英文版);2009-18
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Zhan-qing 1,YANG Hong-wei 2,YE Pei-yan 1,HUANG Qin 1 (1.2nd Hepatitis Department,Shanghai Municipal Infectious Diseases Hospital,Shanghai 200083,China; 2.Dept. of Infectious Diseases,BEN-GANG NAN-DI Hospital,Benxi City,Liaoning 117021,China);Diagnostic Value of Serum Hyaluronic Acid for Hepatic Pathologic Grading and Staging in Patients with Chronic Hepatitis[J];Journal of Tongji University (Medical Science);2003-03
2 Lei Zong, Lun-Gen Lu, Department of Gastroenterology, Renji Hospital, Shanghai Jiao Tong University School of Medicine; Shanghai Institute of Digestive Disease, Shang-hai 200001, China;Research progress on roles of immune factors in development of hepatic fibrosis[J];World Chinese Journal of Digestology;2007-03
3 ZHENG Min, CAI Weiming, WENG Honglei, et al. Institute of Infectious Diseases, First Affiliated Hospital, School of Medicine,Zhejiang University, Hangzhou, Zhejiang 310003,China;ROC curve in evaluation of serum fibrosis index for diagnosing hepatic fibrosis[J];Chinese Journal of Infectious Diseases;2002-04
4 LI Chengzhong, WAN Mobin, ZENG Minde, et al. Changhai Hospital, Second Military Medical University, Shanghai 200433, China [;A preliminary study of the combination of noninvasive parameters in the diagnosis of liver fibrosis[J];Chinese Journal of Hepatology;2001-05
5 CHEN Yong-peng, FENG Xiao-rong, DAI Lin, DING Hong-bing, ZHANG Lian. Department of Infectious Diseases, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China;Screening and evaluation of non-invasive diagnosis markers for compensated liver cirrhosis in patients with chronic hepatitis B[J];Chinese Journal of Hepatology;2003-04
6 CA1 Wei-min, ZHANG Bin-bin, WENG Hong-lei, HU Zhong-rong, LV Jun, ZHENG Mm, LIU Rong-hua. Institute of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China Corresponding author: CAI Wei-min.;The diagnostic value of eight serum indices for liver fibrosis[J];Chinese Journal of Hepatology;2004-04
7 LU Lun-gen, ZENG Min-de. Department of Gastroenterology, Renji Hospital, Shanghai Institute of Digestive Diseases, Shanghai Second Medical University, Shanghai 200001, China;Diagnosis and evaluation of hepatic fibrosis[J];Chinese Journal of Hepatology;2005-08
8 Li Wei-dao,Ou Xiao-juan,Ye Hong-jun, et al .。ChongQing Cancer Institute,Chongqing 630030;CLINICAL EVALUATI0N OF SERUM PROCOLLAGEN TYPE Ⅲ IN THE DIAGNOSIS OF HE- PATIC FIBROSIS:THE ANALYSIS 0F ITS RESUITS OF DETERMINAION IN 1 199 CASES[J];;1995-02
9 Bai Shishan, Li Xiuxia, Yang Shouchang et al. Department of Infectious Diseases, rd Hospital of PLA, Huhehaote 010051;^^ A STUDY OF THE RELATIONSHIP BETWEEN SERUM LEVELS OF HYALURONIC ACID TYPE Ⅲ PROCOLLAGEN, LAMININ, PROLIDASE AND HEPATOFIBROSIS[J];CHINESE JOURNAL OF HEPATOLOGY;1997-02
10 CAI Weimin, ZHENG Min, WENG Honglei, et al The Institute of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China;Determination and significance of serum markers for fibrosis in patients with chronic hepatitis[J];Chinese Journal of Internal Medicine;2001-07
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