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《中国林学(英文版)》 2004-03
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Optimization of the uidA Gene Transfer of Rosa hybrida via Agrobacterium tumefaciens: an Assessment of Factors Influencing the Efficiency of Gene Transfer

Gao Liping Bao Manzhu* Key Laboratory of Horticulture Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, P. R. China  
To develop a transformation protocol of Rosa hybrida Samantha via Agrobacterium tumefaciens, the authors examined the effect of different factors on T-DNA transfer by measuring transient expression levels of an intron-containing -glucuronidase gene. The results indicate that explant, light condition, salt concentration and acetosyringone (AS) concentration in co-culture medium are the most important factors, and factors like co-culture temperature, co-culture period and bacteria density have a strong effect on the growth of bacteria and then T-DNA transfer. Optimized co-cultivation was performed by inoculation of embryogenic callus with bacteria at a density of OD600= 0.50.8 for 20 min and co-culture in darkness under 23 C on medium with 1/2 MS salts and 300 mol稬1 AS for 3 d.
【Fund】: Supported by the National Natural Science Foundation of China (Grant No.30170666)
【CateGory Index】: S685.12
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1 DU Li1,ZENG Xiao-hui1,ZHOU Suo1,BAO Man-zhu2(1 Shool of Life Science and Technology,Nanyang Normal University,Nanyang,He'nan 473000,China;2 Key Laboratory of Horticultural Plant Biology of Ministry of Education,College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China);Transfer of Green Fluorescent Protein Gene into Embryogenic Calluses of Cinnamomum camphora[J];Acta Botanica Boreali-Occidentalia Sinica;2008-03
2 WU Li-jun1,2(1.Fujian Academy of Forestry,Fuzhou,Fujian 350012,China;2.Virginia Polytechnic Institute and State University,Virginia Blacksburg 24061);Study on somatic embryogenesis of elite longleaf pine families[J];Journal of Fujian College of Forestry;2008-01
3 PAN Xiao-hua,HUANG Ru-zhu,ZHU Jin-mao(Bioengineering College,Fujian Normal University,Fuzhou 350007,China);Study on Total DNA Extraction from Cinnamomun Camphora[J];Journal of Fujian Forestry Science and Technology;2004-01
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9 Fan Guoqiang Li Ming He Yaoqing Jiang Jianping (Henan Agricultural University Zhengzhou450002);Somatic Embryogenesis of Platanus orientalis and Its Plantlet Regeneration[J];Scientia Silvae Sinicae;2004-03
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