Plant regeneration of transgenic China Rose (Rosa chinensis Jacq.) from organogenic callus
Chen Ji-ren Liu Rong Chen Shou-yi Wang Hua-fang College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, P. R. China Hunan Biological and Electromechanical Polytechnic, Changsha 410127, P. R. China Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, P. R. China
Different types of explants of China Rose (Rosa chinensis Jacq.) were placed on a Schenk and Hildebrandt (SH) medium containing L-proline and 2,4-dichlorophenoxyacetic acid (2,4-D). Organogenesis was observed on callus induced from both whole leaf and petiole and the high frequency of organogenesis was observed on the whole leaf. Shoot regeneration was obtained via organogenesis. The effects of pH and concentrations of antibiotics on maintenance of organogenesis capacity were investigated in subsequent subcultures. The pH value was found to play a critical role in retaining organogenesis capacity. The binary vector pBI121, carrying the gus gene coding forβ-glucuronidase (GUS) and the nptⅡgene mediated by Agrobacterium tumefaciens, was used for transformation of organogenic callus using 50 mg·L-1 geneticin for selection. Six regenerated lines showed GUS activity, of which five were verified for the presence of nptⅡgene by PCR.
【CateGory Index】： S685.12