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《Chinese Journal of Blood Transfusion》 2003-01
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Genotyping of Duffy blood group and its possible application in HDN prenatal diagnosis

Li Jianping,Liu Xianzhi, Iwamoto S,et al Liaoning Province Blood Center,Shenyang 110044,China  
Objective To evaluate a new molecular method for Duffy blood group genotyping and the possibility to apply this method to prenatal diagnosis of HDN.Methods PCR based restriction fragment length polymorphisms assay was designed to define Duffy blood group,in which the source of template DNA is derived from blood or amniotic fluid.Results Blood DNA samples from 90 unrelated Chinese individuals of Han population in Liaoning province,were analyzed with PCR RFLP and Duffy blood group was genotyped.3 genotypes were detected and the results of genotyping were consistent with those of serology;a DNA sample of amniotic fluid from a Fy(a-b+) pregnant woman,whose husband's Duffy phenotype was Fy(a+b-),was genotyped to be FYAFYB by PCR RFLP.Conclusion Blood and amniotic fluid samples can be correctly genotyped with PCR RFLP assay and the method an be applied to prenatal diagnosis of HDN resulting from anti Fya or anti Fyb.
【Fund】: 辽宁省自然科学基金 (编号 :972 2 3 4)资助项目
【CateGory Index】: R714.5
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