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《Chinese Journal of Blood Transfusion》 2003-03
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Application of Y-chromosome specific short tandem repeat loci in noninvasive prenatal diagnosis

Deng Zhihui, Wu Guoguang, Hao Guiqin ,et al. Shenzhen Institute of Transfusion Medicine, Shenzhen ,518035, China  
Objective To establish a noninvasive method for prenatal genetic analysis by using maternal serum and apply the method in fetal sex determination,paternity testing. Methods Samples of maternal serum from 53 pregnant women (11 to 36 weeks of gestation) were collected. The DNA extracted from each sample was amplified by using"Y-PLEX 6" amplification kit .which enabled the simultaneous analysis of six Y-STR loci including DYS393.DYS19.DYS389 II, DYS390, DYS391 and DYS385. The PCR products were detected by using ABI PrismTM 377 Sequencer and genotyped by related analysis software. Results (1) Y-STR specific alleles were detected in the maternal sera of all 29 mothers bearing male babies. Among the six Y-STR loci,specific alleles were detected in 29/29 at DYS393 locus,in 18/29 at DYS19 locus and in 10/29 at DYS390 locus. (2) Y-STR specific alleles were not detected in maternal sera of 24 pregnant women bearing female babies. (3) According to the presence of specific alleles at DYS393 locus and the value of allelic peak height and peak area, the accuracy of fetal sex determination was 100% . (4)The observed Y-STR alleles of each prenatal specimen from pregnant women with male fetuses were the same as the results of their husbands. Conclusion The assay of highly polymorphic Y-STR genotyping system developed by the authors provided a sensitive, accurate and non-invasive method to prenatal diagnosis. Our results demonstrate that fetal sex can be accurately determined and imply that paternity testing could be performed for pregnant women carrying male fetuses.
【Fund】: 广东省卫生厅科研课题基金(编号A2001642)
【CateGory Index】: R714.5
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【References】
Chinese Journal Full-text Database 8 Hits
1 DENG Zhihui,LI Qian,LIANG Yanlian,et al.Shenzhen Blood Center,Shenzhen 518035,China;Application of MiniSTR in the detection of fetal DNA in maternal plasma[J];Chinese Journal of Blood Transfusion;2008-07
2 DENG Zhihui,LI Dacheng,YU Qiong,et al.Shenzhen Institute of Transfusion Medicine,Shenzhen518035,China;Genetic polymorphisms of 6 Y-chromosome specific STR loci in southern Han population of China and its application in paternity testing[J];Jiangxi Journal of Medical Laboratory Sciences;2004-01
3 Chen Yuekang, Li Qian, LI Dacheng, et al. Shenzhen Blood Center, Shenzhen 518035, China;Study on the genentic polymorphism of 17 Y-chromosome specific STR loci of non-related male individuals in southern Chinese Han population[J];Experimental and Laboratory Medicine;2008-04
4 LI Qian,GAO Su-Qing, LI Heng-Cong1, WANG Da-Ming, ZENG Jian-Qiang, DENG Zhi-Hui Shenzhen Blood Center, Shenzhen 518035, Guangdong Province, China; 1Nanning Central Blood Bank, Nanning 530003, Guangxi Zhuang Autonomous Region, China;Genetic Polymorphism of 9 Y-STR Loci with Short Fragment Size Alleles in Unrelated Male Individuals from Zhuang Ethnic Group[J];Journal of Experimental Hematology;2008-03
5 DENG Zhi-Hui,LI Qian,WU Shuang,LI Da-Cheng. YANG Bao-Cheng Shenzhen Institute of Transfusion Medicine,Shenzhen,518035,Guangdong Province,China;Application of 17 Y-Chromosome Specific STR Loci in Paternity Testing[J];Journal of Experimental Hematology;2008-03
6 DENG Zhi-Hui,WU Guo-Guang, ZHANG Xuan(Shenzhen Institute of Transfusion Medicine, Shenzhen 518035, China);Genetic Polymorphisms of 6 Y-Chromosome Specific STR Loci in the southern Chinese Han Population and Its Application in Forensic Science[J];Hereditas(Beijing);2004-04
7 DENG Zhi-hui,LI Qian,LI Da-cheng,WANG Da-ming,GAO Su-qing,WU Guo-guang. (Shenzhen Institute of Transfusion Medicine,Shenzhen,Guangdong,518035 P.R. China. );The genetic polymorphisms of nine Y-STR loci with short fragment size alleles in southern Chinese Han population and its applicatioin in forensic science[J];Chinese Journal of Medical Genetics;2006-04
8 CHEN Hui-jin 1,PU Hong-wei 2,HU Jia3,WANG Wei3,ZHANG Li-ping1 (1.Biochemistry and Moleculor Biology Section,Foundation Medical College,Xinjiang Medical University; 2.Pathology Section,Foundation Medical College,Xinjiang Medical University;3.Criminal Science and Technology Institute,Xinjiang Bazhou Police);Genetic polymorphism of seventeen Y-chromosomeal STR loci in XinJiang Bazhou Uygur population[J];Journal of Chongqing Medical University;2011-09
【Citations】
Chinese Journal Full-text Database 1 Hits
1 ZHANG Jian,HOU Yiping,TANG Jianpin.( West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan, 610041 P.R.China.E mail:rechtsme@wcums.edu.cn);Polymorphic markers of chromosome Y and their applications for forensic science[J];Chinese Journal of Medical Genetics;2002-05
【Co-citations】
Chinese Journal Full-text Database 1 Hits
1 DENG Zhihui,LI Dacheng,YU Qiong,et al.Shenzhen Institute of Transfusion Medicine,Shenzhen518035,China;Genetic polymorphisms of 6 Y-chromosome specific STR loci in southern Han population of China and its application in paternity testing[J];Jiangxi Journal of Medical Laboratory Sciences;2004-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LIU Chao1, 2, CHEN Ling1, CHEN Xiao-hui2, LIU Chang-hui2, WANG Hui-jun1 1Institute of Forensic Medicine, Southern Medical University, Guangzhou 510515, China; 2Guangzhou Criminal Science and Technology Institute, Guangzhou 510030, China;Polymorphism of 12 Y-STR loci in Guangzhou Han population and its application in forensic medicine[J];Journal of Southern Medical University;2007-05
2 WANG Hui-pin1, LIU Chao2, SUN Hong-yu1 (1. Department of Forensic Biology, Sun Yat-sen University, Guangzhou 510080, China; 2. Guangzhou Criminal Science ﹠ Technology Institute, Guangdong 510030, China);Recent Advancement in MiniSTR Research[J];Journal of Forensic Medicine;2006-02
3 Zhao Ruijun;Zhao Yangxing; Li Jinhua et al(Dept.of Parasitology,Shanxi Medical College,Taiyuan, 030001);TKM──a new method for whole blood DNA preparation[J];;1996-01
4 Chen Hui, Yu Chunying, Yang Qing'en et al Department of Forensic Biology, Faculty of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technologg, Wuhan 430030;Application of STR Loci HumFGA and D3S1359 in Forensic Medicine[J];Acta Universitatis Medictnae Tangji;2002-05
5 Zou Li, Chen Lijuan, Zhu Jianwen Department of Obstetrics and Gynecology, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022;Change of Fetal Nucleated Red Blood Cells in Maternal Blood with Pregnancy-induced Hypertension Syndrome[J];Acta Universitatis Medictnae Tangji;2002-06
6 WU Wei-wei,ZHENG Xiao-ting,PAN Li-peng et al.(Department of Criminal Investigation,Zhejiang provincial public security bureau,Hangzhou 310009,China);A study of polymorphisms of 16 Y-STR loci in Han population in Zhejiang[J];Forensic Science and Technology;2005-05
7 TANG Shuang bo1, GUO Jing yuan1, LIU Chao2, LIANG Shang you2, WANG Sui bao2(1 Faculty of Forensic Medicine,Sun Yat sen University of Medical Sciences,Guangzhou 510089; 2 Institute of Criminal Sciences,Guangzhou 510030, China);Y-DNA STRs Polymorphisms and Haplotypes in Guangzhou Han Population[J];HEREDITAS(BEIJING);2000-01
8 DENG Zhi-Hui,WU Guo-Guang, ZHANG Xuan(Shenzhen Institute of Transfusion Medicine, Shenzhen 518035, China);Genetic Polymorphisms of 6 Y-Chromosome Specific STR Loci in the southern Chinese Han Population and Its Application in Forensic Science[J];Hereditas(Beijing);2004-04
9 Wang Danrui, Cao Shujun, Cui Lijing, et al. Taihe Hospital, Hubei Shiyan 442000;Assessment of Fetal Maturation by Epithelial Growth Factor in Serum of Pregnant Women[J];Chinese Journal of Obstetrics And Gynecology;1998-03
10 ZHU Jianwen, ZOU Li, YANG Yu, et al. Union Hospital, Tong Ji Medical College, Hua Zhong University of Science and Technology, Wuhan 430022,China;Number of Fetal Nucleated Red Blood Cell in Maternal Blood and Placenta in Pregnancies with Intrauterine Growth Retardation[J];Chinese Journal of Perinatal Medicine;2002-02
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 Li Weichun,Wu Rong,Ni Yue,et al Chuzhou Clinical Institute of Anhui Medical University,Chuzhou 239000,China;Extraction of fetal DNA in maternal plasma and detection of short tandem repeat gene[J];Anhui Medical Journal;2010-05
2 HOU Wei-guang1,ZHANG Lei2,YANG Yong-li1,LIU Xue-feng1,YANG Xiao-lin1,REN Fu1(1. Research Institute of Anthropology,Liaoning Medical College,Jinzhou 121000;2.Liaocheng Institute of Criminal Science and Technology,Liaocheng 252000 China);Genetic polymorphisms of 17 Y-STR loci in Han population in the western aera of Shandong province of China[J];Progress of Anatomical Sciences;2011-02
3 Chen Yuekang, Li Qian, LI Dacheng, et al. Shenzhen Blood Center, Shenzhen 518035, China;Study on the genentic polymorphism of 17 Y-chromosome specific STR loci of non-related male individuals in southern Chinese Han population[J];Experimental and Laboratory Medicine;2008-04
4 LI Cheng-tao,LI Li,LIN Yuan,LIU Yan,QUE Ting-zhi,ZHAO Zhen-min(Institute of Forensic Sciences,Ministry of Justice,shanghai 200063,China);Y-STR Genotyping in Forensic Identification Using a 17-Ioci Multiplex PCR Assay[J];Chinese Journal of Forensic Sciences;2006-01
5 ZHANG Xiu-hua1,FAN Xue-hui1,WU Deng-jun2(1.Department of Life Sciences and Technology Xinxiang Medical University,Xinxiang 453003,China;2.College of Animal Science and Technology,Sichuan Agricultural University,Ya'an 625014,China);Genetic Polymorphism of DYS458,DYS534,DYS426 and DYS626[J];Biotechnology;2010-03
6 XU Shi-lun1,3,WANG Yong-yi2,ZHANG Xin-min3,4,HAN Jun3(1 College of Plant Protection,Northwest A & F University,Yangling,Shanxi 712100,China;2 College of Biology and Environmental Science,Jishou University,Jishou,Hunan 416000,China;3 Zhumadian Agricultural College,Zhumadian,He'nan 463000,China 4 College of Economics and Management,Agricultural University of China,Beijing 100094);Genetic law in general populations Ⅱ.Genetic of multiple alleles[J];Journal of Northwest A & F University(Natural Science Edition);2007-05
7 DAI Rong1,2,ZHOU Pin2,SHEN Si-jun1,GAN Shang-quan2,LI Ning3,LIU Shou-ren1,2(1.College of Animal Science and Technology,Shihezi university,Shihizi 832000,China;2.Institute of Animal Science,Xinjiang Agricultural Reclamation Academy,Shihezi 832000,China;3.College of Biological Science and Technology,China Agricultural University,Beijing 100193,China);Parentage identification of sheep from gene transferred somatic cells[J];Animal Husbandry & Veterinary Medicine;2010-11
8 XUAN Zhi-xing, ZHANG Shou-chun (College of Biological science and technology, ShenYang Agriculture University, ShenYang, 110161,China);The research progress of DNA molecular markers on paternity verification of animals[J];Livestock and Poultry Industry;2009-06
9 WANG Ke-jie1,WU Hong-yan1,YANG Wei-hong2,et al(1.Department of Forensic Genetics,Xinxiang Medical College,Xinxiang 453003,China;2.Department of Forensic Genetics,Zhengzhou University,Zhengzhou 450052,China);Fluorescent multiplex amplification and genetic polymorphisms of three Y-chromosomal STRs loci in Han people in Zhengzhou of Henan province[J];Journal of Xinxiang Medical College;2009-05
10 LI Qian,GAO Su-Qing, LI Heng-Cong1, WANG Da-Ming, ZENG Jian-Qiang, DENG Zhi-Hui Shenzhen Blood Center, Shenzhen 518035, Guangdong Province, China; 1Nanning Central Blood Bank, Nanning 530003, Guangxi Zhuang Autonomous Region, China;Genetic Polymorphism of 9 Y-STR Loci with Short Fragment Size Alleles in Unrelated Male Individuals from Zhuang Ethnic Group[J];Journal of Experimental Hematology;2008-03
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 YUAN Yi-Da; JIN Feng; ZHANG Cheng(Institute of Genetics Chinese Academy of Sciences Beijing 100101)Naruya Saitou(National Institute of Geneties Mishima, Japan);The Study of the Distribution of Chinese Surnames and the Diversity of Genetic Population Structure in the Song Dynasty[J];ACTA GENETICA SINICA;1999-03
2 HOU Yiping *, WU Jin, LI Yingbi, ZHANG Ji, ZHANG Sizhong, ZHU Jiayou, M.Prinz.*Institute of Forensic Medicine, West China University of Medical Sciences, Chengdu 610041, P.R.China E mail:rechtsme @ wucms.edu.cn;A preliminary study of human Y chromosome specific short tandem repeat loci[J];CHINESE JOURNAL OF MEDICAL GENETICS;1999-02
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