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《Chinese Journal of Blood Transfusion》 2003-04
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Determination of the numbers of RHD in D antigen positive individuals and nonfunctional RHD allele carriers

Zhou Yiyan,Shao Chaopeng Shenzhen Blood Centere,Shenzhen 518035, China  
Objective To determine the numbers of RHD in D antigen positive individuals Methods The downstream and hybrid Rhesus boxes were amplified and the PCR products were digested by Pst I through a PCR RFLP method in 10 D positive,5 weak D,25 D el phenotype donors and 12 nonfunctional RHD allele carriers by taking 5 D negative samples as a RHD -/RHD - control, which were previously tested by serological and sequencing.Results All 10 D positive individuals were detected RHD +/RHD + homozygotes while 5 weak D samples and 12 nonfunctional RHD allele carriers are RHD +/RHD - heterozygotes.Among 25 D el samples, 9 of them (36%) are RHD +/RHD + homozygotes and 6 of 9 are DCCee, 3 of 9 are DCcee.The rest 16 D el samples are RHD +/RHD - heterozygotes and all are DCcee phenotype.Conclusions The RHD +/RHD + homozygote is the major RHD genotype in D positive Chinese while most of the weak D individuals are heterozygous.Furthermore,there is a high rate of RHD +/RHD + genotype in D el individuals,and the nonfunctional RHD allele carriers are mostly RHD +/RHD - heterozygotes
【Fund】: 深圳市卫生科技计划资助项目 (编号 :深卫科发 2 0 0 2 2 7)
【CateGory Index】: R446.9
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