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《Chinese Journal of Blood Transfusion》 2010-06
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Genetic frequency distribution of human platelet antigens 1—16bw in Nanning population in China

LIAO Yan,SHEN Weidong,LI Lilan,et al.Corresponding author:WU Guoguang. Nanning Institute of Transfusion Medicine,Nanning Blood Center,Nanning 530003,China  
Objective To study the genetic frequency distribution of human platelet antigens in Nanning area,and to provide diagnosis and HPA-matched blood components for patients with alloimmune thrombocytopetic syndromes.Methods A total of 1 500 blood samples from unrelated adults in Nanning were included in the study.Polymerase chain reaction with sequence-specific primers(PCR-SSP) was employed for simultaneous HPA-1 to HPA-16bw genotyping.All PCR amplifications were carried out under identical thermo-cycle condition in 96-well plates containing primer mixture.Results The typing results were available within 4 hours each time after blood samples tested.Among the 16 HPA systems,HPA-15 had the greatest heterozygosity with a gene frequency of 0.252 7,0.500 7 and 0.246 7 for HPA-15a /15a,HPA-15a /15b,HPA-15b /15b,respectively;followed by HPA-3,which showed the heterozygosity with a gene frequency of 0.284 0,0.508 0,and 0.208 0,respectively.For the remaining 14 HPAs,the predominance of a/a homozygosity was noted for HPA-1,-2,-4,-5 and-6,with a frequency ranged from 0.916 7 to 0.999 3.Besides HPA-3b/3b and HPA-15b/15b homozygosity,five cases was detected as HPA-2b/2b homozygosity,and only one case was found as HPA-1b/1b homozygosity in this study.The frequency of a/a homozygosity was 1.000 0 for HPA-7bw to-14bw,-16bw.Conclusion We still have to be alert for the possible events caused by alloantibodies against HPA-1,-2,-3,-5,-6 and-15 in future clinical practice.HPA genotyping may facilitate better diagnosis of alloimmune thrombocytopenia and set up a useful HPA-matched plateletpheresis donor registry,therefore,a more effective platelet transfusion program.
【Fund】: 血小板免疫学诊断技术难题的国际合作研究与应用(08160-05)
【CateGory Index】: R446.6
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