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《Chinese Journal of Blood Transfusion》 2013-12
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Application of nucleic acid test for the blood donors' blood screening in Guangzhou,China

ZHENG Yourong;LIANG Haojian;LI Zhongping;DU Rongsong;HUANG Zhijian;LIN Yashi;LAN Lanyin;WANG Hao;HUANG Aiqiong;FU Yong-shui;Guangzhou Blood Center;  
Objective To investigate the effectiveness and necessity of nucleic acid test for blood screening. Methods Novartis Procleix TIGRIS automatic nucleic acid test and analysis system and PROCLEIX ULTRIO assay were performed to jointly detect HBV,HCV and HIV-1 in 616 705 blood donors. Moreover,nucleic acid identification test was used to identify NAT positive alone samples. Furthermore,HBsAg,anti-HCV, anti-HIV,anti-TP and ALT were test by serological reagent. Results Of 616 705 blood donors samples,1. 62 %( 10 048 / 616 705) were EIA positive and 0. 95%( 5 874 /616 705) were NAT positive. There was a significant difference between EIA positive and NAT positive. Of 594 325 ELISA negative blood donors,0. 22 %( 1 365 /594 325) were NAT positive. Of 1365 only NAT positive blood donors,including 143 HBV DNA positive,1 HCV positive and 1 HIV positive,30. 4%( 415 /1 365) were nucleic acid identification test positive. Of 5 510 two EIA reagent positive blood donors,75. 28% were NAT positive. But only 2. 27%( 103 /4 538) NAT positive was found in one EIA reagent positive blood donors. 14 554 cases of ALT double reagent unqualified samples,NAT positive rate was 2. 31%( 336 /14 554). Among them,14 080 cases of single ALT detection of unqualified specimen,nucleic acid were detected in 2 cases,the positive rate is 0. 01%,and after six months follow-up of donors,NAT test were negative. Conclusion The present risk of transfusion transmitted disease mainly was HBV infection. Nucleic acid test technique was used for blood screening,which will help to shorten the window period of blood transfusion infection and further improve blood safety.
【Fund】: 卫生部行业科研专项(201002005);; 广州市重大科技专项(2010UI-E00661);; 广州市医药卫生重点项目(201102A212010)
【CateGory Index】: R446.6
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