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《Chinese Journal of Blood Transfusion》 2014-01
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Experimental study on the platelet transfusion refractoriness mediated by the anti-CD36:four cases reports in China

WU Guoguang;ZHOU Yan;ZHONG Zhoulin;LI Lilan;LIU Jinlian;SHEN Weidong;Nanning Institute of Transfusion Medicine;  
Objective To confirm experimentally four case of the platelet transfusion refractoriness(PTR) mediated by the anti-CD36 in China.Methods Four patients with clinical PTR were studied.The anti-human platelet antigens(HPA) antibodies and anti-CD36 antibody in the sera of four patients were detected and identified by platelet immuno uorescence test-flow cytometry(PIFT-FC) and monoclonal antibody-specific immobilization of platelet antigens assay(MAIPA).CD36 expression on platelets and monocytes were examined by IFT-FC and monocyte immuno uorescence test-flow cytometry(MIFT).For the confirmation of CD36 deficiency,the CD36 gene of the four patients was sequenced as genomic analysis,and their mutations of the CD36 genes were further verified by DNA-based PCR-SSP assay.Results All four patients were type Ⅰ CD36 deficiency and the anti-CD36 were identified in patients' sera.CD36 mutations identified in 4 patients were 380C T(1 case),329-330 del AC(2 cases) and 1156C T(1 case),respectively.Conclusion The patients with platelet transfusion refractoriness are approved that caused by anti-CD36.Since Chinese populations have a higher proportion of CD36 deficiency,isoimmunization against CD36 should be considered in patients with apparent alloimmune platelet disorders not explained by immunization against HPA and HLA.Such cases should give targeted diagnosis and treatment.
【Fund】: 广西科学研究与技术开发计划项目(主席基金)(08160-05);; 南宁市科学研究与技术开发计划项目(科技惠民重大科技专项)(20133141)
【CateGory Index】: R457.1
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