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《Surface Technology》 2011-01
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Hydrogen Peroxide Biosensor Based on L-cysteine/Multi-walled Carbon Nanotube/Horseradish Peroxidase Modified Glod Electrode

MA Mei-ping,CAO Jian-ming(School of Environmental Science and Public Health,Wenzhou Medical College,Wenzhou 325000,China)  
Horseradish peroxidase(HRP) was selected as a model enzyme to fabricate a novel biosensor based on the L-cysteine,mutli-walled carbon nanotube.The electrochemical characteristics of the electrode were characterized by cyclic voltammetry.And the effects of pH and temperature of phosphate buffer solution on the response to H2O2 were also studied.At last,the reproducibility and selectivity of the modified electrode were investigated in detail.The results show that the modified electrode exhibites wide linear range,lower detection limit and good stability,as well as certain anti-interference ability.
【Fund】: 温州市科技计划项目(Y20100360);; 2010年浙江省高校优秀青年教师资助项目(浙教办高科〔2010〕175号);; 温州市科技局项目(Y20080102)
【CateGory Index】: TP212.3
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