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《Journal of Beijing Agricultural Vocation College》 2008-05
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DNA Template Preparation and Technology System Building for AFLP Analysis of Rosa Rugosa.

Li Yushu1,Zhang Qixiang2(1.Beijing Agricultural Vocation College,Beijing 102442,China;2.Beijing Forestry University,Beijing 100083,China)  
Extraction of DNA with phenol-cholorofore method from Rosa rugosa leaves was dealt with and AFLP finger printing in 37 R.rugosa cultures and 5 Rosa davurica were established.Clear and high resolution DNA finger prints were obtained,which mena success in DNA extraction,restriction-ligasereaction,pre-amplification and staining techniques.A foundation for the establishment of gene pool,selection and breeding of eminent cultivars and geneic relaionshpi in R.rugosa was laid.
【Fund】: “十五”国家科技攻关资助项目(2004BA525B11)的部分成果
【CateGory Index】: S685.12
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