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Extraction of Chlorogenic Acid from Flos Lonicerae and Its Antibacterial Activity

WANG Hong-Jun, WU Guo-Juan, JIANG Hong et al(Beijing Agricultural College, Beijing 102206)  
Optimization of the extracting method of Chlorogenic Acid from Flos Lonicerae was studied by three different methods of ultrasonic extraction, sorbitic extraction, and improved method of sorbitic extraction. The quantative and qualitative determination of Chlorogenic acid in Flos Lonicerae has been carried out by two different ways of Ultraviolet and Visible Spectrophotometric(UV) and High Performance Liquid Chromatographic(HPLC). Inhibition test against pathogenic bacteria has been done with Flos Lonicerae ethanol legging and Chlorogenic acid. The results showed that Chlorogenic acid tiptop absorb-apex was 327 nm, and improved method of sorbitic extraction was superior remarkably to other methods (P0.01), and the content of Chlorogenic acid of witch eventual producetion was disdilled by the improved method of sorbitic extraction was 64.01%. So the improved method of sorbitic extraction is the best way. The antibacterial action in vitro to pathogenic bacteria caused mastitis with Flos Lonicerae ethanol legging showed that Staphylococcus aureus was extremely sensitive, and Streptococcus uberis, Clostridium aerogenes capsulatus were highly sensitive, and Streptococcus agalactiae, Streptococcus dysgalactiae were moderately sensitive. The minimal inhibitory concentration (MIC) of Chlorogenic acid performed by the tube and plate method to Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis, Clostridium aerogenes capsulatus were 1.58 g/L, 3.16 g/L, 3.16 g/L, 6.32 g/L, 1.58 g/L respectively and minimal bactericidal concentration (MBC) were 1.58 g/L, 6.32 g/L, 3.16 g/L, 6.32 g/L, 3.16 g/L
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