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《Journal of Beijing Agricultural College》 2006-03
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Antifungal Substance from Bacillus sp.BJ-6 and Its Antagonism against Plant Pathogens

REN Zheng-guang~1,LIU Yuan~2,LIU Su-hua~1,CHEN Yong~1,ZHANG Zhiyong~1,WEI Yan-min~(1*)(1.Department of Plant Science and Technology,Beijing Agricultural College,Beijing 102206,China;2.National Institute of Metrology P.R.China,Beijing 100013,China)  
The strain BJ-6 of Bacillus sp.was isolated from the rhizosphere of apricot trees in Mentougou,Beijing,China,and showed strong antifungal activity to six tested species of plant pathogens,i.e.Physalospora piricola,Alternaria mali,Valsa mali,A.brassicae,Botrytis cinerea and Verticillium dahliae.This study showed that the culture liquid of BJ-6 strain preserved its inhibitory activity to tested pathogenic fungi when autoclaved at 121℃ for 20 min at pH≤7.0,and was not stable when treated at the same temperature at pH≥9.0.The crude antifungal proteins were separated from BJ-6 fermented solution by ammonium sulphate precipitation at different saturations from 20% to 90%,among which the crude antagonistic proteins precipitated by a saturation of 30%~40% had the highest inhibiting activity.The crude proteins solution which had an UV absorption peak at 274 nm had inhibiting activity against plant pathogenic fungi and brought the A.brassicae hyphae abnormal.
【Fund】: 北京市教委资助项目(项目编号:KM200510020001 KM200410020008)
【CateGory Index】: S476
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