Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Beijing University of Agriculture》 2008-02
Add to Favorite Get Latest Update

An Effective Method for Isolation of High-quality Total RNA from Fruit Pulps

ZHU Xiao-qin,JING Xiao-le,FENG Yong-qing,SHEN Yuan-yue*(Depertment of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206,China)  
Although isolation of total plant RNA has become a conventional technique,owing to the particularity of fruit material,there is not yet ideal method for the isolation of total RNA.Based on previous CTAB method,an common method for isolation of total RNA from fruit was discussed in order to lay the foundation on fruit development molecular biology.Total RNA was successfully isolated from fresh apple,peach,strawberry and grape fruits by the effective CTAB method modified with a 16% of β-Mercatoethanol and 3% of PVP in extraction buffer,1/3(V/V)of 5 mol/L KAC(pH4.8)in supernatant.The isolated RNA was assayed by ultraviolet spectrophotometry,electrophoresis,reverse transcription and RT-PCR and showed that the modified CTAB protocol was preferred method for isolation of high-quality total RNA from fruit pulp.Soybean pollen-tube.
【Fund】: 北京市自然科学基金资助项目(项目编号:6082005);; 北京市属市管高校人才强教计划资助项目(项目编号:6082005PXM2007014207);; 北京农学院引进人才项目(项目编号:60820059997116038)和重点项目(项目编号:60820052026016005)
【CateGory Index】: S66
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 1 Hits
1 Jin Xiaolei Shen Yuanyue Liu Min Chao Huijuan Dong Qinghua Beijing University of Agriculture,Department of Plant Science and Technology,Beijing,102206;Cloning and Analysis of Polyphenol Oxidase Gene in Strawberry[J];Molecular Plant Breeding;2008-05
Chinese Journal Full-text Database 2 Hits
1 Zhuang Junping1 Su Jing2 Chen Weixin1*1 Key Lab of Fruit and Vegetable Storage of Guangdong Province, Horticulture College of Sourth China Agricultural University, Guangzhou, 510642; 2 Life Science College of Zhongshan University, Guangzhou, 510275;An Effective Method for High-quality RNA Isolation from Banana Fruit[J];Molecular Plant Breeding;2006-01
2 YAO Yu-xin, ZHAO Ling-ling, HAO Yu-jin, ZHAI Heng*(College of Horticultural Science and Engineering,Shandong Agricultural University, Tai'an, Shandong 271018 China);Effective extraction of total RNA in apple flesh with improved hot borate method[J];Journal of Fruit Science;2005-06
Chinese Journal Full-text Database 10 Hits
1 CHEN Chang-bao,ZHU Shu-hua,ZHOU Jie(College of Chemistry and Material Science,Shandong Agricultural University,Taian 271018,China);Improved CTAB Method for Total RNA Extraction of Mature Feicheng Peach Fruit[J];Shandong Agricultural Sciences;2009-05
2 LI Na et al(Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants,Mininstry of Education,Beijing Forestry University,Beijing 100083);Comparative Study on Methods of RNA Isolation from Phloem of Anthocephalus chinensis[J];Journal of Anhui Agricultural Sciences;2008-11
3 LI Jing et al(Xinjiang Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Urumqi,Xinjiang 830011);Study on Total RNA Extraction and Anti-transcription Activity from Transgenic Wheat Leaves with Total DNA of Leymus racemosus(Lam.) Tzvel in mRNA Differential Displaying Technology[J];Journal of Anhui Agricultural Sciences;2009-11
4 GAN De-fang1,WANG Xiao-jun2(1.School of Horticulture,Anhui Agricultural University,Hefei 230036;2.Agency of Shanghua Street of Lanxi City in Zhejiang Province,Lanxi 321100);Study on methods of extracting total RNA from Chinese cabbage leaves[J];Journal of Anhui Agricultural University;2007-02
5 Li Hao1,2 Zhang Pingping2,3 Zha Xiangdong1 He Zhonghu2,4 Xia Xianchun2* 1 School of Life Science Anhui University,Anhui Key Laboratory of Eco-engineering and Bio-technique,Hefei,230039;2 Institute of Crop Science,National Wheat Improvement Center,Chinese Academy of Agricultural Sciences,Beijing,100081;3 College of Plant Science and Technology,Huazhong Agricultural University,Wuhan,430070;4 CIMMYT China Office,Beijing,100081;A Method for Rapid Isolation of High-Quality Total RNA from Wheat Seeds[J];Molecular Plant Breeding;2006-06
6 Wu Guoqiang Xi Jiejun Zhou Xiangrui Ma Qing Wang Suomin School of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730000;An Improved Method for Extracting Total RNA from Leaves and Roots of the Succulent Xerophyte Zygophyllum xanthoxylum[J];Molecular Plant Breeding;2008-01
7 CHEN Di,FU Xian-ying,YIN Xiao-min,ZHENG Rong-yi(College of Environment and Plant Protection,SCUTA,Danzhou 571737,China);Assessment of several extraction methods of total RNA from fruits[J];Guangdong Agricultural Sciences;2007-11
8 SUN De-quan1,2,GUO Qi-gao1,HU Yu-Lin2,XIE Jiang-hui2(1.School of Horticulture and Landscape,Southwest University,Chongqing 400716,China;2.Southern Asia Subtropical Crop Reseach Institute,CATAS,Zhanjiang 524091,China);Extraction of total RNA from banana with improved Trizol method[J];Guangdong Agricultural Sciences;2009-05
9 GAN Guo-ying,QI Yan-xiang,PU Jin-ji,ZHANG Xin,XIE Yi-xian(Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Danzhou 571737,China);Improved CTAB method for extracting high quality total RNA from banana leaves[J];Guangdong Agricultural Sciences;2009-07
10 LI Zhi-qiang,LI Ying,TAO Jian-min,QIAO Yu-shan,QU Shen-chun,ZHANG Zhen(College of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu 210095 China);Extraction and quality analysis of total RNA from different tissues of different fruits[J];Journal of Fruit Science;2008-05
Chinese Journal Full-text Database 2 Hits
1 Dong Chen1,2* Diao Ying1,2,3* Wang Manling1,2 Zhu Honglin1,2 Li Guolin 1,2 Zhou Mingquan2 Hu Zhongli1,2** 1 Key Lab of MOE for Plant Developmental Biology College of Life Science,Wuhan University,Wuhan,430072;2 Lotus Center,Wuhan University,Wuhan,430072;3 Department of Life Science,Chongqing University of Arts and Science,Yongchuan,400068;Molecular Cloning and Analysis of Polyphenol Oxidase Gene in Lotus[J];Molecular Plant Breeding;2006-06
2 HUANG Jian-an1,HUANG Yi-huan1,LUO Jun-wu1,LI Jia-xian2,GONG Zhi-hua1,LIU Zhong-hua1(1.Key Laboratory of Tea Science of Ministry of Education,HNAU,Changsha 410128,China;2.Tea Research Institute,Guang Dong Academy of Agricultural Sciences,Yingde,Guangdong 513042,China);Identification of single nucleotide polymorphisms (SNPs) in polyphenol oxidase gene in tea plant(Camellia sinensis)[J];Journal of Hunan Agricultural University(Natural Sciences);2007-04
【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
1 ;A Method for Extraction of RNA From Mulberry[J];Acta Sericologica Sinica;2004-02
2 Xu Changjie,Chen Kunsong,Zhang Bo ,Wang Qianjie,and Ye Weijia (Department of Horticulture,Huajiachi Campus,Zhejiang University/The State Agricultural Ministry's Laboratory of Horticultural Plant Growth,Develop ment &Biotechnology,Hangzhou,Zhejiang310029China);A Study on Methods for RNA Extraction From Citrus Tissues[J];Journal of Fruit Science;2004-02
3 CHENG Shui-yuan1,2 , CHEN Kun-song1*, DU He-wei2, XU Wen-ping1(1Fruit Science Institute, Zhejiang University, Hangzhou, Zhejiang 310000 China; 2The key Discipline group of Garden Plant and Ornamental Horticulture, Yangtze University, Jingzhou, Hubei 434025 China);Total RNA extraction of Ginkgo biloba leaves[J];Journal of Fruit Science;2005-04
4 HU Wei-rong 1,2, ZHU Xi-ru1, WANG Zheng-xun1, ZHANG Zhao-qi2, JI Zuo-liang2 (1.School of Biology and Chemistry Engineering,Guangzhou University,Guangzhou 510405, China; 2.College of Horticulture,South China Agricultural University,Guangzhou 510642, China);Advances of research on postharvest and storage physiology of banana fruit[J];Journal of Guangzhou University(Natural Science Edition);2003-03
5 JIANG Jian xiong,ZHANG Tian zhen * (National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, China);Extraction of Total RNA in Cotton Tissues with CTAB-acidic Phenolic Method[J];Acta Gossypii Sinica;2003-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved