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《Journal of Beijing University of Agriculture》 2010-03
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Stomach action of osthole and its effect on two enzyme activities of Plutella xylostella larvae

MENG Lin-lin,WANG Jin-zhong,ZHANG Zhi-yong,SUN Shu-ling,ZHANG Min-zhao,ZHANG Peng-fei,DING Bao-li,PU Yuan-yuan(Department of Plant Science & Technology,Beijing University of Agriculture,Beijing 102206,China)  
Stomach action of osthole to 3rd instar larvae of Plutella xylostella and its effects on activities of glutathione-S-transferase(GSTs)and acetylesterase(AChE) were studied in the laboratory.The results showed that the osthole had stomach action on the Plutella xylostella larvae.At a concentration of 2 mg/mL,the corrected mortality of the larvae was 37% at 12 h after osthole application,while the corrected mortality of larvae was 93% at 72 h.The LC50 for the 3rd instar larvae was 1 521.25 mg/mL,865.93 mg/mL and 1 521.25 mg/mL at 24 h,48 h,and 72 h,respectively.The activities of GSTs and AChE of larvae changed with concentrations modification of osthole,the highest GSTs activity of larvae treated with the dosage of 125×10-3 mg/mL of osthole was 209.15 U·mg-1prot min-1 at 12 h,while the highest AChE activities was 0.648 1 U·mg-1 prot·min-1at 4 h.While the GSTs and AChE activities decreased significantly at 24 h.These results suggested osthole could influence GSTs and AChE activities of Plutella xylostella larvae.
【Fund】: “大学生科学研究与创业行动计划”项目;; 北京市教育委员会项目(KM200610020007 PXM2008_014207_055164);; 北京市自然基金(5043026);; 北京市科委项目(Z080005032508017);; 北京市市管高校人才强教计划资助项目;; 国家自然科学基金(30971938)
【CateGory Index】: S482.3
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