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《Journal of Beijing University of Agriculture》 2013-02
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Rapid regeneration of GlycyrrhizauralensisFisch

HUANG Yujie,WANG Dan,WANG Xu,SONG Juan,ZHAO Dongli *(Medical College of Dalian University,Dalian 116622,China)  
Glycyrrhiza uralensis is a medicinal plant that has been widely used in Chinese folk medicine for relieving fever,detoxifcation,reducing phlegm,stopping cough,tonifying spleen and stomach,etc.Tissue culture and plantlets regeneration of this plant were investigated in this work.The results showed that the cotyledon and hypocotyl are suitable explants for inducing callus among the 5explants including cotyledon,hypocotyl,radicle,young stem and leaf.MS+6-BA 1.0 mg/L+ NAA1.5mg/L is the best medium for callus formation,and also the buds formation from explants of axillary bud and stem tip.1/2MS+NAA 0.05mg/L is the best root induction medium among the 3hormones IAA 、 IBA and NAA,and rooting rate is about 80%.
【Fund】: 大连大学本科生创新基金项目
【CateGory Index】: S567.239
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