BASIC RESEARCH ON PHYSICOCHEMICAL PROPERTIES OF THREE TRANSFER FACTORS
Wang Guilan, Zhao Chi, Wang Li, Zhang Pinglan(Deft.of Immunol., Dept. of Microbiol. , Pharm Factory of N.Bethune Univ.of Med.Sci.)
Three transfer factors were prepared from pig,human and bovine spleens by homogenization and dialysis. Under the same conditions, the physicochemical properties of these 3 TFs were determined by means of determination of nucleo-tide and peptide contents, HPRC, analysis of free a mi no acids, absorbance, etc.HTF and PTF absorbance of E 260/E280 was more than 2, the strongest absorption spectrum for HTF and PTF was at a wave length of 250 ±2 nm,and these two TFs were separated into 1 -2 distinct fractions by HPRC, whereas BTF absorbance of E260/E280 was about 1 , the absorption spectrum for BTF was at a wave length of 268 ±2 nm, and this TF was separated into 5 - 6 distinct fractions by HPRC.