Relationship of Cardioprotection of Ischemic Preconditioning and Opioid Receptor
FU Shao peng 1,JIANG Yi zhong 1,ZHANG Wen jie 2 (1. Dept.of Cardiovascular Surgery, China Japan Union Hospital,Jilin University,Changchun 130031,China;2. Dept.of Physiology, School of Basic Medical Sciences,Jilin University)
Objective:To observe the salutary effects of ischemic preconditioning (IPC) on heart after global ischemia/reperfusion (GI/R) and discover the potential mechanism of this phenomenon. Methods:The adult mice hearts were perfused in Langendorff mode. 24 hearts were randomly divided into four groups: Control Group,Ischemic Group,IPC Group,NAL+IPC Group. Hemodynamic parameters and CK release were observed in the 10th after reperfusion. Results:Following sustained GI/R, in IPC Group, CK release was significantly reduced and Hemodynamic parameters were better. Naloxone infusion blocked the protection from IPC in isolated hearts including CK release. Conclusion:In the isolated mouse heart,two cycles of IPC with 5 min ischemia and 5 min reperfusion are necessary to induce the cardioprotection in the mouse heart, which can be blocked by the naloxone infusion, The cardioprotection induced by IPC is associated with opioid receptor.
【CateGory Index】： R541