Relationship of Cardioprotection of Ischemic Preconditioning and Opioid Receptor
FU Shao peng 1,JIANG Yi zhong 1,ZHANG Wen jie 2 (1. Dept.of Cardiovascular Surgery, China Japan Union Hospital,Jilin University,Changchun 130031,China;2. Dept.of Physiology, School of Basic Medical Sciences,Jilin University)
Objective:To observe the salutary effects of ischemic preconditioning (IPC) on heart after global ischemia/reperfusion (GI/R) and discover the potential mechanism of this phenomenon. Methods:The adult mice hearts were perfused in Langendorff mode. 24 hearts were randomly divided into four groups: Control Group,Ischemic Group,IPC Group,NAL+IPC Group. Hemodynamic parameters and CK release were observed in the 10th after reperfusion. Results:Following sustained GI/R, in IPC Group, CK release was significantly reduced and Hemodynamic parameters were better. Naloxone infusion blocked the protection from IPC in isolated hearts including CK release. Conclusion:In the isolated mouse heart,two cycles of IPC with 5 min ischemia and 5 min reperfusion are necessary to induce the cardioprotection in the mouse heart, which can be blocked by the naloxone infusion, The cardioprotection induced by IPC is associated with opioid receptor.