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《Journal of Jilin University of (Medicine Edition)》 2005-01
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Biological features of hMSC and effects of bFGF on its proliferation

CHANG Ying 1, QI Xin 2,BU Li-sha 3,XU Zhong-Xin 1, YANG Hong 1 (1. Department of Neurology,China-Japan Union Hospital ,Jilin University, Changchun 130031,China; 2.Department of Orthopedics, First Hospital ,Jilin University, Changchun 130021,China; 3.Department of Central Laboratory, China-Japan Union Hospital,Jilin University,Changchun 130021,China)  
Objective To study the growth pattern and surface makers of adult human mesenchymal stem cells (hMSC) and the influences of bFGF on the proliferation of hMSC.Methods The growth curves of the hMSC were drawn; FACS was used to detect surface antigenic markers. Cell growth pattern and its response to bFGF were evaluated by MTT test. Results The latent phase of the primary culture cells was 5-9 days, followed by 11-16 days of logarithmical proliferation; the plateau began at day 18-20. The subcultures showed 12-24 hours of latent phase, followed by 7-10 days of logarithmical proliferation, then entered the plateau (11-13 days). The hMSC expressed positive CD44 unanimously, but don′t expressed CD45. Low concentration of bFGF (10 μg·L -1) could stimulate the proliferation of hMSC significantly (P0.05); while 25-100 μg·L -1 of bFGF could inhibit the ALP activity. Conclusion hMSC can be expanded in vitro rapidly. The effect of bFGF on hMSC proliferation is in positive dose-effect manner.
【Fund】: 吉林省科技厅资助课题 (2 0 0 30 336 - 5 )
【CateGory Index】: R329.2
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【References】
Chinese Journal Full-text Database 2 Hits
1 ;Application of bFGF/collagen sustained release sponge in apexification[J];Journal of Jilin University(Medicine Edition);2006-03
2 Chen Bing1,Yin Yan-qing1,Ke Jun-long2,Zou Xin-hui1,Peng Hao1,Tan Shan-feng1,Xu Zhi-en2 1Department of Neurosurgery,2Department of Neurology,Affiliated Hospital of Guangdong Medical College,Zhanjiang 524001,Guangdong Province,China;Ligustrazine induces rat bone morrow mesenchymal stem cells to differentiate into neuron-like cells:Screening of the optimal inductive concentration[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2010-06
【Citations】
Chinese Journal Full-text Database 1 Hits
1 HUANG Lan feng 1, SUN Shu dong 1, LIU Jian guo 2, XU Xin xiang 2 (1 Dept. of Orthopaedics, The Second Hospital,Jilin University, Changchun 130041,China; 2. Dept.of Orthopaedics,The First Hospital, Jilin University);Osteogenic Stimulation and Identification of Mesenchymal Stem Cells in Rabbits[J];Journal of Jilin University of (Medicine Edition);2003-06
【Co-citations】
Chinese Journal Full-text Database 1 Hits
1 FENG Min,XIA Yonghui,CUI Wen,LIN Yongmin,YANG Qingying,RU Yongxin Institute of Hematology & Blood Diseases Hospital,Chinese Academy of Medical Sciences,Tianjin 300020,China;Proliferation and Differentiation Characteristics of Bone Marrow Mesenchymal Stem Cells in Patients with Hematological Diseases[J];Tianjin Medical Journal;2009-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHAO Dong-hai~1,WANG Yue-zeng~2,GUO Xin~2,LI Yu-lin~ 2 (1. Department of Vaccine Center, Jilin University, Changchun 130012, China;2. ME Key Laboratory of Pathobiology,School of Basic Medical Sciences,Jilin University, Changchun 130021,China);Isolation and identification of human mesenchymal stem cells[J];Journal of Jilin University of (Medicine Edition);2005-02
2 YANG Yan~1,WANG Juan~2,CHEN Xiao~1,KANG Li-hua~1 (1. Department of Hematology and Oncology,First Hospital,Jilin University,Changchun 130021,China; 2.Department of Oncology,People′s Hospital,Jilin Province,Changchun 130021,China);Culture and biological characteristics of adult human bone marrow-derived mesenchymal stem cells in vitro[J];Journal of Jilin University of (Medicine Edition);2005-02
3 JIANG He-bi, ZHUO Ben-hui, DAI Ying, QU Ping, LI Ting-yu (Department of Child Health Care, College of Pediatrics, Chongqing University of Medical Sciences, Institute of Nutrition Research for Children, Chongqing 400014, China);bFGF promotes differentiation of mesenchymal stem cells into neurons and the related mechanisms[J];Acta Academiae Medicinae Militaris Tertiae;2005-02
4 ZHENG Lei WANG Qian WEI Kuan-hai Pei Guo-xian (Department of Orthopedics and Traumatology. Clinical Laboratory Nanfang Hospital, First Military Medical University. Guangzhou 510515. China);Biological effects of basic fibroblast growth factor on bone marrow stromal cells[J];JOURNAL OF FIRST MILITARY MEDICAL UNIVERSITY;2000-02
5 Liu Jinbao, Dong Xiaoxian, Dong Yanxiang, et al. Department of Pathophysiology, Guangzhou Medical College, Guangzhou 510182;Bone mesenchymal stem cells differentiate into neuron-like cells with chinese angelica[J];Guangdong Medical Journal;2003-05
6 HE Hui-hua 1, WANG Gui-fang 1, LENG Shui-long 2, LIU Jin-bao 1, DONG Xiao-xian 1, DONG Wei-hua 1 (1.Department of Pathophysiology, 2.Department of Anatomy, Guangzhou Medical College, Guangzhou 510182);Modified Isolation and Cultivation of Human Mesenchymal Stem Cells in Vitro[J];Academic Journal of Guangzhou Medical College;2004-04
7 LIU Fuyun 1) , SHI Hong 1) , QI Yan 2) ,WEN Jianguo 2) ,XU Shang'en 1) ,YANG Qizheng 1) 1)Department of Pediatric Surgery, the Third Affiliated Hospital, Zhengzhou University Zhengzhou 450052 2)Urodynamic Center,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052;Urodynamic examination and surgery treatment for infants and children with tethered cord syndrome[J];Journal of Henan Medical University;2003-02
8 RUAN Yi\|wen, WANG Chuan\|en, TONG Jian\|er, YAO Zhi\|bin * (Department of Anatomy and Brain Research,Sun Yat\|sen University of Medical Sciences,Guangzhou\ 510089,China) \;[;BEHAVIORAL EFFECT OF TRANSPLANTATION OF THE NEURAL STEM CELLS MODIFIED WITH GENE OF NGF OR GDNF ON AD MODEL RAT[J];Acta Anatomica Sinica;2002-02
9 SA Ya-lian, LI Hai-biao *(Department of Histology and Embryology of Sun Yat-Sen University of Medical Sciences, Guangzhou 510089,China);DIFFERENTIATION OF MESENCHYMAL STEM CELLS INTO NEURON- LIKE CELLS WITH LIGUSTRAZIN HYDROCHLORIDE[J];Acta Anatomica Sinica;2003-05
10 HAN Li-chi,HU Jing,QI Meng-chun,et al. Department of Oral and maxillofacial Surgery,West China College of Stomatology, Sichuan University, Chengdu 610041;An Experimental Study of Isolation and Pluripotential of Rat Bone Marrow Stem Cells[J];Journal of Comprehensive Stomatology;2004-02
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 JIAO Yue1,2,WANG Xiao-jie1,3,ZHANG Rui1,2,CAI Min-qian1,LI Ting-ting1,GAO Li-chang1, WANG Shan-shan4,LI Hai-yan1,LI Xiao-kun1,3(1.Engineering Research Center of Bioreactor and Pharmaceutical Development,Ministry of Education, Jilin Agricultural Univerity,Changchun 130118,China;2.College of Traditional Chinese Medicine, Jilin Agricultural Univerity,Changchun 130118,China;3.Key Laboratory of Biotechnology Pharmaceutical Engineering,Zhejiang Province,Department of Pharmacy,Wenzhou Medical College,Wenzhou 325000,China; 4.Harbin Peiqilong Biological Pharmaceutical Co.,Ltd,Harbin 150090,China);Establishment of method for detection of biological activity of biological protein sponge of basic fibroblast growth factor and stability evaluation[J];Journal of Jilin University(Medicine Edition);2011-01
2 LIU Jing;YANG Sheng-hui;LI Jin-lu;Capital Medical University School of Stomatology;;Inhibition of periodontal pathogens by immobilized minocycline prepared with collagen in vitro[J];Beijing Journal of Stomatology;2014-03
3 CHEN Wen-ping1,JIANG Gui-lin1,WANG Chao1,2,NI Xue-wen1,LI Dong-sheng1,JIANG Fa-tang1,2(1.School of Biological Engineer.,Hubei Univ.of Technology,Wuhan 430068,China; 2.Wuhan Licheng Biotechnology Co.,Ltd.,Wuhan 430068,China);Advances in the application of biopolymer sustained/controlled release carriers[J];Strait Pharmaceutical Journal;2009-11
4 LI Yu-bing,HUANG Zheng-de(TCM University of Hunan,Changsha,Hunan,410208,China);Progress of intervention mechanism of Chinese medicine on cardiovascular diseases treated with stem cell transplantation[J];Journal of Traditional Chinese Medicine University of Hunan;2011-07
5 Yanqing Yin1,Bing Chen1,Junlong Ke2,Wei Mo1,Xinhui Zou1,Yuansheng Liang1 1Department of Neurosurgery,Affiliated Hospital of Guangdong Medical College,Zhanjiang 524001,Guangdong Province,China 2Department of Neurology,Affiliated Hospital of Guangdong Medical College,Zhanjiang 524001,Guangdong Province,China;Xuefuzhuyu injection induces neuronal differentiation of rat bone morrow mesenchymal stem cells[J];中国神经再生研究(英文版);2011-03
6 WANG Yu1,WEI Shu-yan2,SHI Xiao-qin1,WANG Yan1, ZHANG Ming-yan1,CHEN Yu-min1(1.School of Basic Medical Sciences,Hebei University,Baoding,Hebei 071000,P.R.China; 2.Department of Neurology,the First Center Hospital of Baoding, Baoding,Hebei 071000,P.R.China);Research of Cytidine Triphosphate induce adult rat bone mesenchymal stem cells into Neuron-like cells[J];China Journal of Modern Medicine;2011-17
7 Yang Ling1, Zhang Zuo-tao1, Zhang Li-hua2, Lu Ying1 1Department of Human Anatomy, Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Guizhou Province, China; 2Department of Human Anatomy and Embryology, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, Sichuan Province, China;Yougui Wan combined with basic fibroblast growth factors induce the differentiation of rat bone marrow stromal cells into neuron-like cells[J];Chinese Journal of Tissue Engineering Research;2012-32
8 Xie Xing-wen1, Hou Fei-yi2, Li Ning2, Li Sheng-hua1, Song Min2 1Pharmaceutical Research Institute of Gansu Province, Lanzhou 730050, Gansu Province, China; 2Gansu University of Traditional Chinese Medicine, Lanzhou 730000, Gansu Province, China;Hoechst33342 for labeling rat bone marrow mesenchymal stem cells[J];Chinese Journal of Tissue Engineering Research;2012-49
9 Huang Cheng;Yang Jian-dong;Feng Xin-min;Xu Wei;Li Yi-nan;Xiao Hai-xiang;Gu Jia-xiang;Medical School of Yangzhou University;Northern Jiangsu People's Hospital;;Proliferation and differentiation of mesenchymal stem cells modified with glial cell line-derived neurotrophic factor[J];Chinese Journal of Tissue Engineering Research;2013-45
10 FANG Jian-kang;ZHOU Yi-ping;LI Ma-lin;School of Traditional Chinese Medicine,Yunnan University of Traditional Chinese Medicine;School of Pharmaceutical Science & Key Laboratory of Pharmacology for Natural Products,Kunming Medical University;;Research progress on effects of traditional Chinese medicines on proliferation,apoptosis and differentiation of bone marrow mesenchymal stem cells[J];China Journal of Chinese Materia Medica;2014-15
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