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《Journal of Jilin University of (Medicine Edition)》 2005-02
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Isolation and identification of human mesenchymal stem cells

ZHAO Dong-hai~1,WANG Yue-zeng~2,GUO Xin~2,LI Yu-lin~ 2 (1. Department of Vaccine Center, Jilin University, Changchun 130012, China;2. ME Key Laboratory of Pathobiology,School of Basic Medical Sciences,Jilin University, Changchun 130021,China)  
Objective To establish a method to isolate and identify human mensenchymal stem cells(HMSC). Methods The HMSC were isolated and cultured in vitro by applying methods of density-grad centrifugal and anchoring screening. The immunocytochemistry, cytochemistry, and electron microscopy were used to identify HMSC cultured in vitro. Results The HMSC have particular phenotype:CD29 and CD44 were positive,CD34 and CD45 were negative.PAS was positive,and SB was negative.HMSC were infantile under electron microscope. Conclusion The HMSC can be isolated with density-grad centrifugal and anchoring screening. The method to identify HMSC with immunocytochemistry, cytochemistry and electron microscopy is effective.
【Fund】: 教育部博士学科点专项基金资助课题 (2 0 0 2 0 1830 6 4 )
【CateGory Index】: R329.2
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