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《Journal of Jilin University of (Medicine Edition)》 2005-02
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Effects of Panaxadiol Saponins on expressions of CD14 and NF-κB in lung of endotoxic shock rats

LI Lu~ 1,2, LIU Xi-chun~1,YU Zhen-xiang~ 1,3,LI Yang~1, ZHAO Dan~1, ZHANG Jian~1, ZHAO Xue-jian~ 1 (1. Department of Pathophysiology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China; 2. Department of Preventive Medicine, School of Basic Medical Sciences, Changchun of College TCM, Changchun 130021,China;3. Departmentof Respiratory Medicine, First Hospital, Jilin University, Changchun 130021, China)  
Objective To explore the molecular mechanism of protective effects of Panaxadiol Saponins (PDS) on lung tissues of endotoxic shock rats. Methods Wistar rats were randomly divided into control group (CTRG), lipopolysaccharide (LPS) shock group (LPSG), LPS shock+dexamethasone group (DEXG) and LPS shock+PDS group (PDSG), respectively. Endotoxin shock models were duplicated by injection of LPS (4 mg·kg~ -1). The histological changes of the lung tissues stained with hematoxylin and eosin (HE) among four groups were compared through microscopic examination. Meanwhile, the expressions of CD14 and IκBα mRNA were measured by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and the expressions of CD14 and NF-κB proteins were determined by Western blotting assay. Results ①The histological and pathological changes: in LPSG, there was the effusion of a large amount of lymphocytes, mono-macrophages and neutrophils around the alveolar and vascular walls; incrassated alveolar septums and bronchiole walls were caused by hyperemia ,edema and infiltration of pulmonary interstitial;focal atrophia and formation of pneumatocele could be observed; only small amount of alveolus containing air could be found; however, the lung pathologic changes in DEXG and PDSG were significantly slighter than those in LPSG, and they merely showed slight effusion of inflammatory cells, and the content of air in alveolus was near to that in CTRG. ②The expressions of CD14 mRNA and protein in LPSG were significantly higher than those in CTRG(P0.05), while those in DEXG and PDSG were close to those in CTRG(P0.05), significantly lower than those in LPSG(P0.05). The expression of lung IκBα mRNA in LPSG was significantly lower than those in CTRG(P0.01), while those in DEXG and PDSG were close to that in CTRG(P0.05) and higher than that in LPSG(P0.05 and P0.01). The expression of NF-κB P65 protein in LPSG was significantly strengthened than that in CTRG(P0.05), while those in PDSG and DEXG were lower than that in LPSG(P0.05), close to that in CTRG(P0.05). Conclusion LPS activates the signaling pathway mediated by LPS receptors, the decreasing of IκBα mRNA expression and the increasing of CD14 and NF-kB expressions are the molecular mechanism for the lung tissue injuries, while the opposite changes in PDSG reveal the molecular mechanism of their protective effects on cells and tissues; PDS has the same effects with DEX to protect against the lung tissue injuries of endotoxic shock in rats.
【Fund】: 吉林省科技厅自然科学基金资助课题 (2 0 0 30 5 5 1- 3) ;; 吉林省科技厅自然科学基金攻关课题 (2 0 0 30 5 0 1)
【CateGory Index】: R285
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【References】
Chinese Journal Full-text Database 5 Hits
1 WANG Zhi1,JI Guo-yi1,LI Yang1,ZHU Shi-gong2,ZHAO Xue-jian1,SUN Lian-kun1,KANG Jin-song1(1.Department of Pathophysiology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China; 2.Department of Physiology,Health Science Center,Beijing University,Beijing 10083,China);Effects of Dex on TLR4,IκBα and IL-18 mRNA expressions of cortex in endotoxic shock rats[J];Journal of Jilin University(Medicine Edition);2007-05
2 YU Zhen-xiang, ZHAO Heng, PENG Li-ping (Department of Respiratory Medicine,First Hospital,Jilin University,Changchun 130021,China);Protective effects of PDS on oxidative damage in lung of hemorrhage-LPS two hits rats[J];Journal of Jilin University(Medicine Edition);2008-04
3 CHEN Hong-mei1,LI Yan-jing1,WANG Jin-ke2,LI Ping11Department of Pharmacognosy, China Pharmaceutical University,Nanjing 210038,China;2State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China;Advances in regulation of nuclear factor-κB activation by traditional Chinese medicines[J];Pharmaceutical and Clinical Research;2008-02
4 YU Zhen-xiang1,LIU Xi-chun2,ZHAO Xue-jian2(1Department of Respiratory Medicine,First Hospital,2Department of Pathophysiology,School of Basic Medical Science,Jilin University,Changchun 130021,China);Panaxadiol saponins alleviates acute lung injury in the two-hit rat model with hemorrhagic shock and lipopolysaccharide[J];Chinese Journal of Pathophysiology;2008-12
5 YU Zhen-Xiang, ZUO Meng-Hua, PENG Li-Ping, et al.Department of Respiratory Medicine, First Hospital, Jilin University, Changchun 130021, Jilin, China;The effect of panaxadiol saponin on the expressions of TLR4 and IL-18 in lung tissues of rats with acute lung injury induced by hemorrhagic shock-endotoxin two-hit[J];Chinese Journal of Gerontology;2007-20
【Citations】
Chinese Journal Full-text Database 2 Hits
1 CUI Xin ming 1,LI Yan ru 1,LU Wen wei 2,CUI Li 1,LIU Jie 2 TIAN Jian ming 3,LI Long yun 3,YE Jin mei 3,ZHANG Zhi wei 3,DOU Ying △ (1. Dept.of Electron Microscopy,School of Basic Medical Sciences, Jilin University, Changchun 130021,China;2. Dept.of Pharmacology,School of Basic Medical Sciences, Jilin University;3. Institute of Chinese Traditional Medicine and Herbs,Jilin Province);Protective Effect of Rg_2 on Myocardium of Dog with Acute Cardiogenic Shock[J];Journal of Jilin University of (Medicine Edition);2003-04
2 Xu Lin, Zhao Xue-Jian, et al Departments of Pathophysiology and Biochemistry, Norman Bethune University of Medical Science, Changchun;Effect of panaxadiol saponins on the monoaminemediator and MAO in hemorrhagic shock dogs[J];Chinese Journal of Pathophysiology;1990-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Liu Yuanyuan, Lu Meide, Zhao Xuejian Wang Zhongshan, Zhao Dan(Dept. of Infect. Dis, First Teaching Hosp., Dept. of Pathophysiology);EFFECT OF PANAXADIOL SAPONIN ON 5-HT IN RATS WITH ENDOTOXIC SHOCK[J];;1991-04
2 LU Wen-wei1, LIU Jie1, ZHAO Li-juan1,TIAN Jian-ming2, LI Long-yun2, YANG Shi-jie 1 (1. Department of Pharmacology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China; 2.Academy of Traditional Chinese Medicine and Materia,Jilin Prorince, Changchun 130021,China);Effects of ginsenoside Rg_2 on hemodynamics and oxygen metabolism in narco-canine with acute cardiogenic shock[J];Journal of Jilin University of (Medicine Edition);2004-04
3 XIN Ying,NI Jin-Song,WANG Xin-Rui,SHI Bo, WANG Yue-Hui, WU Jia-Xiang (Department of Pathology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China);Inhibitory effect of 20(S)-ginsenoside Rg_3 on B16 melanoma metastasis[J];Journal of Jilin University of (Medicine Edition);2004-04
4 WANG Zhi, LI Jin-cheng, LIU Xiao, LIU Xi-chun, ZHAO Xue-jian, SUN Lian-kun  (Department of Pathophysiology, School of Basic Medical Sciences, Jilin University,Changchun 130021, China);Effects of PDS on ⅠκBα mRNA expression of cortex in endotoxic shock rats[J];Journal of Jilin University of (Medicine Edition);2005-01
5 WANG Qiu-jing~1, LIU Jie~1, LIU Fen~1,LI Yang~2(1. Experimental Center of Functional Sciences, School of Basic Medical Sciences, Jilin University,Changchun 130021,China;2. Department of Pathophysiology, School of Basic Medical Sciences, Jilin University,Changchun 130021,China);Protective effects of PDS on acute cardiogenic shock in dogs[J];Journal of Jilin University of (Medicine Edition);2005-04
6 ;Clinical effects of Carvedilol on dilated cardiomyopathycongestive heart failure[J];Journal of Jilin University of (Medicine Edition);2005-04
7 WANG Zhan-feng~1,LUO Yi-nan~(1*),HONG Xin-yu~1,JU Yan~1,FU Shuang-lin~1,GUO Hai-tao~1,LI Ye~1,HAN Hai-ling~2,SUN Yu-qin~2(1.Department of Neurosurgery,First Hospital,Jilin University,Changchun 130021,China;2.Department of Cardio-Cerebral and Vascular Medicine,Affiliated Hospital,Medical College,Beihua University,Jilin 132011,China);Inhibitory effect of ginsenoside-Rh_2 on glioma cells cultivated in vitro[J];Journal of Jilin University(Medicine Edition);2006-04
8 HU Cui-hua~1,XU Hua-li~1,YU Xiao-feng~1,QU Shao-chun~1,SUI Da-yuan~(1*),CHEN Yan-ping~2,MA Xing-yuan~2(1.Department of Pharmacology,School of Pharmacy,Jilin University,Changchun 130021,China; 2.Department of Nature Medical Chemistry,School of Chemistry,Jilin University,Changchun 130021,China);Effects of Panaxadiol Saponins on blood glucose and lipid metabolism in experimental hyperglycemia of type 2 diabetes mellitus rats[J];Journal of Jilin University(Medicine Edition);2006-06
9 LIU Wei, WANG Yan-Chun, FAN Hong-Yan, SHEN Nan, REN KuangSchool of Basic Medicine, Jilin Medical College, Jilin 132013, China;Effects of ginsenoside Rb_1 on behaviors and memory of lead-exposed mice[J];Journal of the Fourth Military Medical University;2009-13
10 LIU Wei,WANG Yan-chun,FAN Hong-yan,SHEN Nan,REN Kuang Deptartment of Pharmacology,School of Basic Medical Sciences,Jilin Medical College,Jilin 132013,China);Effects of ginsenoside Rb_1 on content of lead in bone and learning and memory in mice exposure to lead[J];Journal of Jilin University(Medicine Edition);2009-05
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 CHEN Li, WU Wei, ZHENG You-liang(Agronomy College,Sichuan Agricultural University,Yaan 625014,China);HPLC Analysis on Dissociative Amino Acids of Houttuynia[J];Amino Acids & Biotic Resources;2004-01
2 Gu Jianzhong, Ye Jing, Zhang Yuan, et al Zhejiang College of TCM,Hangzhou (310053);The Effect of Dachengqi Decoction On The ARDS Rat’s Blood Coagulation System and Platelet[J];;2006-01
3 WAN Shan Xia 1,HUA Jing 1,WANG Wan Wan 2,ZHANG Shu Ping 1 ( 1Beijing Agricultural College,Beijing 102206; 2Beijing Biochemical Phamaceutical Factory,Beijing 100075);Research Status of Trypsin Inhibitor in Different Fields[J];Journal of Beijing Agricultural College;2003-02
4 WANG Qiu-jing~1, LIU Jie~1, LIU Fen~1,LI Yang~2(1. Experimental Center of Functional Sciences, School of Basic Medical Sciences, Jilin University,Changchun 130021,China;2. Department of Pathophysiology, School of Basic Medical Sciences, Jilin University,Changchun 130021,China);Protective effects of PDS on acute cardiogenic shock in dogs[J];Journal of Jilin University of (Medicine Edition);2005-04
5 CHEN Jian-ming,WU Xue-xiang,JIANG Yao-guang,et al. (Department of Cardiothoracic Surgery,Institute of Surgery Research,Daping Hospital, Third Military Medical University,Chongqing 400042,China);Role of protease-antiprotease imbalance in pulmonary contusion in rabbits[J];Journal of Traumatic Surgery;2005-02
6 CHEN-Chen,JIN Yu Department of Pediatrics,First Hospital of Lanzhou University,Lanzhou 730000,China;Effects of oxymatrine on expression of nuclear factor kappa B in kidneys of rats with adriamycin-induced chronic renal fibrosis[J];Journal of Southern Medical University;2007-03
7 XIA Qi-zhong, ZHANG Ming-ju(Huanggang Technical College, Huanggang, Hubei 438002, China);Plant Resistance Genes to Insects and Their Applications[J];Journal of Ezhou University;2005-03
8 HE Tai-ping, HE Zhen-hui, MO Li-er, LIANG Nian-ci (Department of Biochemistry, Guangdong Medical College , Zhanjiang 524023, China);Effects of cantharidin on the expression of NF-κB(P65) and Smad3 genes in cells of ovarian carcinoma cell line HO-8910PM with highly metastatic potential[J];Journal of Guangdong Medical College;2005-02
9 Wang Jiming,Kong Kangmei,Qi Weili,et al.Department of Orthopaedics,the Second Affiliated Hospital,Shantou University Medical College,Shantou,515041;Effects of Salvia Miltiorrhiza on Nuclear Factor-κB Activity in Rats with Acute Traumatic Spinal Cord Injury[J];Chinese Journal of Bone and Joint Injury;2007-04
10 Ren Xinyu, Ruan Qiurong, Zhu Dahe, Zhu Min, Qu Zhiling, Lu Jun (Department of Pathology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China);EFFECT OF TETRAMETHYLPYRAZINE ON NF-κB ACTIVATION AND BMP-2 EXPRESSION IN RAT VASCULAR SMOOTH MUSCLE CELIS[J];Chinese Journal of Histochemistry and Cytochemistry;2003-03
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 YOU Min-Sheng, WEN Jin-Kun, HAN Mei.Basic College, Hebei Medical University, Shijiazhuang 050051, Hebei, China;Effect of Tongxinluo supermicropowder on left ventricular remodeling and the changes of myocardium pathology and mitochondria super-microcosmic structure in rats with acute myocardial infarction[J];Chinese Journal of Gerontology;2009-06
2 ZHOU Guang-ju,ZHANG Mao,GAN Jian-xin,XU Shao-wen,JIANG Guan-yu (Department of Emergency Medicine,The Second Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310009,China. );Evaluation of the inflammatory response in two-hit acute lung injury model of rats using [~(18)F] FDG microPET[J];Chinese Journal of Pathophysiology;2009-07
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 LIU Jie, LV Wen-wei, ZHANG Zhi-wei, LI Long-yun, JIN yi;Effect of ginsenoside Rg2 on acute cardiogenic shock in dogs[J];Chinese Journal of Pathophysiology;2001-09
2 LU Jie1, LU Wen-wei1, TIAN Jian-ming2, LI Long-yun2 (1.Norman Bethune University of Medical Sciences ,Changchun 130021, Jilin, Chin a; 2.Academy of Traditional Chinese Medicine and Materia Jilin Prorince,Changchun 130021, Jilin, China);Effect of Rg_2 on Hemodynamics of Hemorrhagic Shock and its A ntioxidation in Dogs[J];China Journal of Chinese Materia Medica;2001-08
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