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《Journal of Jilin University of (Medicine Edition)》 2005-02
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Culture and biological characteristics of adult human bone marrow-derived mesenchymal stem cells in vitro

YANG Yan~1,WANG Juan~2,CHEN Xiao~1,KANG Li-hua~1 (1. Department of Hematology and Oncology,First Hospital,Jilin University,Changchun 130021,China; 2.Department of Oncology,People′s Hospital,Jilin Province,Changchun 130021,China)  
Objective To establish a method to isolate and culture the bone marrow-derived mesenchymal stem cells (BM-MSCs) in vitro and investigate their biologic characteristics. Methods The culture of mesenchymal stem cells was initiated from bone marrow low-density mononuclear cells separated by Pecoll centrifugation and maintained in low-glucose DMEM with 10% fetal calf serum. The cell growth pattern was evaluated by trypan blue exclusion. The cell cycle and surface antigenic features were analyzed by flow cytometry. Results The BM-MSCs were positive for CD29,CD44,CD166,HLA-ABC and negative for CD34, CD45,and HLA-DR. Cell cycle analysis showed that 12.8% of them was in S phase and 76.4% of them was in G 0/G 1 phase. The cells had a population doubling time of 2-3 days. Conclusion BM-MSCs are a homogenous monoclone population of cells with unique growth phenotype characteristics.
【Fund】: 长春市科委资助课题 (0 4 - 0 7SF- 0 81)
【CateGory Index】: R329.28
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