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《Journal of Jilin University of (Medicine Edition)》 2005-05
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Comparison of staining methods in evaluation of sperm morphology

GAO Jiu-chun,WANG Rui-xue,XU Zong-ge,ZHENG Hai-zheng,LIU Yuan,LIU Rui-zhi~(Department of Cell Biology,School of Basic Medical Sciences, Jilin University,Changchun 130021,China)  
Objective To study the effects of Modified Papanicolaou stain,Wright-Giemisa stain and(Coomassie) brilliant blue stain on the rate of morphogically normal sperm and the rate of acrosome intactness.Methods Semen smears from 55 male infertility patients were stained according to Wright-Giemisa,Modified Papanicolaou and Coomassie brilliant blue methods.The rate of morphologically normal sperm and the rate of acrosome intactness were evaluated.Results ①No significant differences were found in the percentage of morphologically normal sperm between the three staining methods(P0.05).Comparing with Modified Papanicolaou stain and Coomassie brilliant blue stain,the rate of Neck/Midpiece defect sperm,as well as tail defect sperm,significantly decreased after Wright-Giemisa stain(P0.05).②No significant difference was observed in the rate of sperm with type I acrosome and the rate of sperm with type Ⅳ acrosome between the three staining methods(P0.05).However,the rate of sperm with type Ⅱ or Ⅲ acrosme was higher using Modified Papanicolaou stain than using the other two stains(P0.05).③The significantly positive correlationship was found in the rate of normal morphologically sperm(r=0.535 P0.01,r=0.601,P0.01,r=0.329,P0.05),as well as in the rate of acrosome intactness((r=0.604,) P0.01,r=0.682,P0.01,r=0.446,P0.01),among the three staining methods.Conclusion The three staining methods stain would be reliable and applicable to detect the percentage of human morphologically normal sperm and the rate of acrosome intactness.Among the three staining methods,Modified Papanicolaou stain would be better to be used in examing the percentage of human morphologically normal sperm,and Wright-Giemisa stain would be more suitable for detecting the rate of acrosome intactness.
【Fund】: 吉林省科技厅资助课题(20030433)
【CateGory Index】: R446.19
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