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《Journal of Jilin University(Medicine Edition)》 2006-02
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Experimental study on microencapsulated NGF expressing NIH3T3 cells on repair of peripheral nerve defect in rats

ZHAO Jing-guo~1,ZHANG Ju~1,GAO Feng-tong~(1),SUN Hong-bin~1,WANG Wei~2(1.Department of Hand Surgery,China-Japan Union Hospital,Jilin University,Changchun 130031,China;2.Biomedical Material Engineering Group,Department of Biotechnology,Institute of Chemistry and Physics of Dalian,Chiniese Academy of Sciences,Dalian 116023,China)  
Objective To investigate the effects of microencapsulated NGF expressing NIH3T3 cells on the repair of peripheral nerve defect in rats.Methods Thirty SD male rats with 10 mm defect of sciatic nerve were randomly divided into three groups(n=10).Group A: The nerve defect was bridged with heterogeneous nerve,the neural regeneration room was formed and filled with microencapsulated NGF expressing NIH3T3 cells.Group B: Autogenous nerve grafting was performed.Group C: The nerve defect was bridged with heterogeneous nerve,the neural regeneration room was formed and filled with microencapsulated NIH3T3 cells.The walking track analysis,electrophysiological and morphological observation were performed 12 weeks after operation.Results There were no significant differences of sciatic nerve function index,nerve concluctive velocity,histological changes of regenerative nerve,maturation degree of regenerative nerve fiber between group A and group B 4,8,12 weeks after operation;group A and B were surperior to group C(P0.05).Conclusion There is good biocompatibility between the microencapsules and peripheral nerve tissue of rat in vivo.The microencapsulated NGF expressing NIH3T3 cells is good bridge and can promote regeneration of peripheral nerve.
【Fund】: 吉林省科技厅科技发展项目(20030544-1)
【CateGory Index】: R651.3
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