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《Journal of Jilin University(Medicine Edition)》 2006-06
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Antiherpesvirus activity in vitro and immunoregulative effect of ginsenoside Rg3

MEI Shao-lin~1,YUAN Hong-yan~1,CHANG Ya-ping~(1*),LI Ping-ya~2,LU Dan~2(1.Department of Immunology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China;2.Department of New Drugs,Institute of Frontier Medical Sciences,Jilin University,Changchun 130021,China)  
Objective To investigate the inhibitory effect of ginsenoside Rg3 on herpes simplex virus(type 1(HSV-1) in vitro and) its immunoregulative effect.Methods Crystal violet staining was used to observe the inhibitory effect of Rg3 on the cytopathic effect caused by HSV-1.MTT colorimetry was used to detect the influence of Rg3 on the proliferation of Vero cells and mouse splenocytes.The mouse splenocytes were induced with Rg3,and the activity of IL-2 was detected by lymphoblast proliferation assay and IFN-γ by cytopathic effect inhibition.(Results The) A_(570) values of cytopathic effect in the experimental group were significantly higher than those in the control group when doses of Rg3 were 1.56-25 mg·L~(-1) in vitro(P0.05).The A_(570) values of proliferation effect on normal Vero cells and mouse splenocytes had no significant difference between the experimental group and control group when doses of Rg3 were lower than 25 mg·L~(-1)(P0.05).The secretary levels of IL-2 and IFN-γ in experimental group were significantly higher than those in the control group in vitro when doses of Rg3 were(3.13-6.25)mg·L~(-1) (P0.05).Conclusion Rg3 in some extent could inhibit HSV-1 in vitro and has no poisonous effect on normal Vero cells and mouse splenocytes.Rg3 could significantly stimulate the secretion of IL-2 and(IFN-γ,)thereby participate immunoregulative effect.
【Fund】: 吉林省科技厅科技发展计划项目资助课题(20055142)
【CateGory Index】: R285.5
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