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《Journal of Jilin University(Medicine Edition)》 2007-05
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Effect of high concentration glucose on AQP4 expression in rabbit retina Müller cell cultured in vitro

TIAN Kuo1,NI Jin-song1,WANG Xin-rui1,CHEN Di1,WU Jia-xiang1,ZHAO Xue-jian2,YANG Bao-xue2(1.Key Laboratory of Pathobiology,Ministry of Education,School of Basic Medical Sciences,Jilin University,Changchun 130021,China;2.Department of Pathophysiology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China)  
Objective To observe the effects of high concentration glucose on aquaporin 4(AQP4)expression in rabbit retina Müller cell cultured in vitro.Methods The rabbit retinal Müller cells were cultivated in vitro under the condition of 30,40,50 mmol·L-1 high concentration glucose.The AQP4 expression was detected by immunocytochemical staining,FACS and RT-PCR after cultivated for 1,3,and 5 d.Results Green fluorescence of AQP4 expression of Müller cells in high glucose groups was weaker than that in control group(P0.01).FACS results indicated that the position of wave crest advanced obviously and AQP4 expression deseased(P0.01).With the increasing of glucose concentration and prolongation of time,the extent of AQP4 expression was gradually weakened.RT-PCR results revealed that on the condition of 50 mmol·L-1 glucose for 3 d,AQP4 mRNA expression was weaker than that in control group(P0.01).Conclusion High concentration glucose could desease the AQP4 expression of cultured retinal Müller cells.With the increasing of glucose concentration and prolongation of time,the AQP4 expression deseases gradually and it may be a protective response in diabetic retinopathy DR.
【Fund】: 吉林省科技厅科技发展计划项目资助课题(200505141)
【CateGory Index】: R774.1
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